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Title: Development of an adaptable coherent x-ray diffraction microscope with the emphasis on imaging hydrated specimens

Abstract

This paper describes the development of a versatile coherent x-ray diffraction microscope capable of imaging biological specimens in solution. The microscope is a flexible platform accommodating various conditions, from low vacuum (10{sup −2} Pa) to helium gas filled ambient pressure. This flexibility greatly expands the application area, from in situ materials science to biology systems in their native state, by significantly relaxing restrictions to the sample environment. The coherent diffraction microscope has been used successfully to image a yeast cell immersed in buffer solution. We believe that the design of this coherent diffraction microscope can be directly adapted to various platforms such as table top soft x-ray laser, synchrotron x-ray sources, and x-ray free electron laser with minor relevant adjustments.

Authors:
 [1]; ; ; ; ; ; ;  [1];  [1]
  1. RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo, Hyogo 679-5148 (Japan)
Publication Date:
OSTI Identifier:
22224093
Resource Type:
Journal Article
Journal Name:
Review of Scientific Instruments
Additional Journal Information:
Journal Volume: 84; Journal Issue: 11; Other Information: (c) 2013 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0034-6748
Country of Publication:
United States
Language:
English
Subject:
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; BIOLOGY; DESIGN; FREE ELECTRON LASERS; HELIUM; IMAGES; MATHEMATICAL SOLUTIONS; MICROSCOPES; SOFT X RADIATION; SOLUTIONS; SYNCHROTRONS; X-RAY DIFFRACTION; X-RAY SOURCES; YEASTS

Citation Formats

Nam, Daewoong, Department of Physics, Pohang University of Science and Technology, Pohang 790-784, Park, Jaehyun, Shimada, Hiroki, Kim, Sangsoo, Kim, Sunam, Kohmura, Yoshiki, Ishikawa, Tetsuya, Song, Changyong, Gallagher-Jones, Marcus, and Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB. Development of an adaptable coherent x-ray diffraction microscope with the emphasis on imaging hydrated specimens. United States: N. p., 2013. Web. doi:10.1063/1.4828656.
Nam, Daewoong, Department of Physics, Pohang University of Science and Technology, Pohang 790-784, Park, Jaehyun, Shimada, Hiroki, Kim, Sangsoo, Kim, Sunam, Kohmura, Yoshiki, Ishikawa, Tetsuya, Song, Changyong, Gallagher-Jones, Marcus, & Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB. Development of an adaptable coherent x-ray diffraction microscope with the emphasis on imaging hydrated specimens. United States. https://doi.org/10.1063/1.4828656
Nam, Daewoong, Department of Physics, Pohang University of Science and Technology, Pohang 790-784, Park, Jaehyun, Shimada, Hiroki, Kim, Sangsoo, Kim, Sunam, Kohmura, Yoshiki, Ishikawa, Tetsuya, Song, Changyong, Gallagher-Jones, Marcus, and Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB. 2013. "Development of an adaptable coherent x-ray diffraction microscope with the emphasis on imaging hydrated specimens". United States. https://doi.org/10.1063/1.4828656.
@article{osti_22224093,
title = {Development of an adaptable coherent x-ray diffraction microscope with the emphasis on imaging hydrated specimens},
author = {Nam, Daewoong and Department of Physics, Pohang University of Science and Technology, Pohang 790-784 and Park, Jaehyun and Shimada, Hiroki and Kim, Sangsoo and Kim, Sunam and Kohmura, Yoshiki and Ishikawa, Tetsuya and Song, Changyong and Gallagher-Jones, Marcus and Institute of Integrative Biology, University of Liverpool, Liverpool L69 7ZB},
abstractNote = {This paper describes the development of a versatile coherent x-ray diffraction microscope capable of imaging biological specimens in solution. The microscope is a flexible platform accommodating various conditions, from low vacuum (10{sup −2} Pa) to helium gas filled ambient pressure. This flexibility greatly expands the application area, from in situ materials science to biology systems in their native state, by significantly relaxing restrictions to the sample environment. The coherent diffraction microscope has been used successfully to image a yeast cell immersed in buffer solution. We believe that the design of this coherent diffraction microscope can be directly adapted to various platforms such as table top soft x-ray laser, synchrotron x-ray sources, and x-ray free electron laser with minor relevant adjustments.},
doi = {10.1063/1.4828656},
url = {https://www.osti.gov/biblio/22224093}, journal = {Review of Scientific Instruments},
issn = {0034-6748},
number = 11,
volume = 84,
place = {United States},
year = {Fri Nov 15 00:00:00 EST 2013},
month = {Fri Nov 15 00:00:00 EST 2013}
}