KOTOBUKI-1 apparatus for cryogenic coherent X-ray diffraction imaging

Journal Article · Sun Sep 15 00:00:00 EDT 2013 · Review of Scientific Instruments

DOI:https://doi.org/10.1063/1.4822123· OSTI ID:22220351

Nakasako, Masayoshi; Takayama, Yuki; Oroguchi, Tomotaka; Sekiguchi, Yuki; Kobayashi, Amane ^[1]; Shirahama, Keiya ^[1]; Yamamoto, Masaki; Hikima, Takaaki; Yonekura, Koji; Maki-Yonekura, Saori; Kohmura, Yoshiki; Inubushi, Yuichi ^[2]; Takahashi, Yukio; Suzuki, Akihiro ^[2]; Matsunaga, Sachihiro; Inui, Yayoi ^[3]; Tono, Kensuke; Kameshima, Takashi; Joti, Yasumasa ^[4]; Hoshi, Takahiko ^[5]

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1. Department of Physics, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522 (Japan)
2. RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo, Hyogo 679-5148 (Japan)
3. Department of Applied Biological Science Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510 (Japan)
4. Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo, Hyogo 679-5198 (Japan)
5. Kohzu Precision Co., Ltd., 2-6-15 Kurigi, Aso-ku, Kawasaki, Kanagawa 215-8521 (Japan)

We have developed an experimental apparatus named KOTOBUKI-1 for use in coherent X-ray diffraction imaging experiments of frozen-hydrated non-crystalline particles at cryogenic temperature. For cryogenic specimen stage with small positional fluctuation for a long exposure time of more than several minutes, we here use a cryogenic pot cooled by the evaporation cooling effect for liquid nitrogen. In addition, a loading device is developed to bring specimens stored in liquid nitrogen to the specimen stage in vacuum. The apparatus allows diffraction data collection for frozen-hydrated specimens at 66 K with a positional fluctuation of less than 0.4 μm and provides an experimental environment to easily exchange specimens from liquid nitrogen storage to the specimen stage. The apparatus was developed and utilized in diffraction data collection of non-crystalline particles with dimensions of μm from material and biological sciences, such as metal colloid particles and chloroplast, at BL29XU of SPring-8. Recently, it has been applied for single-shot diffraction data collection of non-crystalline particles with dimensions of sub-μm using X-ray free electron laser at BL3 of SACLA.

• OSTI ID: 22220351
• Journal Information: Review of Scientific Instruments, Vol. 84, Issue 9; Other Information: (c) 2013 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA); ISSN 0034-6748
• Country of Publication: United States
• Language: English

Related Subjects

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY
CHLOROPLASTS
COLLOIDS
COOLING
CRYOGENICS
EQUIPMENT
FLUCTUATIONS
FREE ELECTRON LASERS
LIQUIDS
METALS
NITROGEN
PARTICLES
SPRING-8 STORAGE RING
X RADIATION
X-RAY DIFFRACTION
X-RAY DIFFRACTOMETERS
X-RAY LASERS