Sun light mediated synthesis of gold nanoparticles as carrier for 6-mercaptopurine: Preparation, characterization and toxicity studies in zebrafish embryo model

Journal Article · Sat Sep 15 00:00:00 EDT 2012 · Materials Research Bulletin

DOI:https://doi.org/10.1016/J.MATERRESBULL.2012.06.015· OSTI ID:22215702

Ganeshkumar, Moorthy ^[1]; Sastry, Thotapalli Parvathaleswara ^[2]; Sathish Kumar, Muniram ^[3]; Dinesh, Murugan Girija ^[4]; Kannappan, Sudalyandi ^[5]; Suguna, Lonchin ^[1]

---

1. Department of Biochemistry, Central Leather Research Institute, Council of Scientific and Industrial Research, Chennai 600020 (India)
2. Bioproducts Laboratory, Central Leather Research Institute, Chennai 600020 (India)
3. Department of Pharmaceutics, Anna University, Trichy, Tamilnadu (India)
4. Thanthai Hansroever College, Perambalur, Tamilnadu (India)
5. Central Institute of Brackish Water Aquaculture, Chennai 600028 (India)

Highlights: ► Gold nanoparticles prepared using eco-friendly method with good in vitro stability. ► Can be used as drug delivery system. ► Did not show any toxicity in zebrafish embryo. ► More toxic to cancer cells when compared to N-Au-Mp and Mp. -- Abstract: The objective of this study is to synthesize green chemistry based gold nanoparticles by sun light irradiation method. The prepared gold nanoparticles (AuNPs) were modified using folic acid and then coupled with 6-mercaptopurine. These modified nanoparticles were used as a tool for targeted drug delivery to treat laryngeal cancer. In the present study, novel bionanocomposites containing nutrient agar coated gold nano particles (N-AuNPs) coupled with 6-mercaptopurine (drug) (N-AuNPs-Mp), folic acid (ligand) (N-AuNPs-Mp-Fa) and rhodamine (dye) (N-AuNPs-Rd), a fluorescent agent, were prepared and characterized by IR, UV, TEM, Particle size analysis and in vitro stability. The toxicity and fluorescence of N-Au was studied using zebrafish embryo model. The in vitro cytotoxicity of free Mp, N-Au-Mp and N-Au-Mp-Fa against HEp-2 cells was compared and found that the amount of Mp required to achieve 50% of growth of inhibition (IC{sub 50}) was much lower in N-Au-Mp-Fa than in free Mp and N-Au-Mp.

• OSTI ID: 22215702
• Journal Information: Materials Research Bulletin, Vol. 47, Issue 9; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0025-5408
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
77 NANOSCIENCE AND NANOTECHNOLOGY
ABSORPTION SPECTROSCOPY
EMBRYOS
FLUORESCENCE
FOLIC ACID
IN VITRO
INFRARED SPECTRA
IRRADIATION
MERCAPTOPURINE
NANOSTRUCTURES
NEOPLASMS
PARTICLE SIZE
PARTICLES
STABILITY
SYNTHESIS
TOXICITY
TRANSMISSION ELECTRON MICROSCOPY