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Title: CXCL2 synthesized by oral squamous cell carcinoma is involved in cancer-associated bone destruction

Abstract

Highlights: Black-Right-Pointing-Pointer Oral cancer cells synthesize CXCL2. Black-Right-Pointing-Pointer CXCL2 synthesized by oral cancer is involved in osteoclastogenesis. Black-Right-Pointing-Pointer CXCL2-neutralizing antibody inhibited osteoclastogenesis induced by oral cancer cells. Black-Right-Pointing-Pointer We first report the role of CXCL2 in cancer-associated bone destruction. -- Abstract: To explore the mechanism of bone destruction associated with oral cancer, we identified factors that stimulate osteoclastic bone resorption in oral squamous cell carcinoma. Two clonal cell lines, HSC3-C13 and HSC3-C17, were isolated from the maternal oral cancer cell line, HSC3. The conditioned medium from HSC3-C13 cells showed the highest induction of Rankl expression in the mouse stromal cell lines ST2 and UAMS-32 as compared to that in maternal HSC3 cells and HSC3-C17 cells, which showed similar activity. The conditioned medium from HSC3-C13 cells significantly increased the number of osteoclasts in a co-culture with mouse bone marrow cells and UAMS-32 cells. Xenograft tumors generated from these clonal cell lines into the periosteal region of the parietal bone in athymic mice showed that HSC3-C13 cells caused extensive bone destruction and a significant increase in osteoclast numbers as compared to HSC3-C17 cells. Gene expression was compared between HSC3-C13 and HSC3-C17 cells by using microarray analysis, which showed that CXCL2 gene wasmore » highly expressed in HSC3-C13 cells as compared to HSC3-C17 cells. Immunohistochemical staining revealed the localization of CXCL2 in human oral squamous cell carcinomas. The increase in osteoclast numbers induced by the HSC3-C13-conditioned medium was dose-dependently inhibited by addition of anti-human CXCL2-neutralizing antibody in a co-culture system. Recombinant CXCL2 increased the expression of Rankl in UAMS-32 cells. These results indicate that CXCL2 is involved in bone destruction induced by oral cancer. This is the first report showing the role of CXCL2 in cancer-associated bone destruction.« less

Authors:
 [1]; ;  [1];  [1];  [2];  [3]; ; ; ;  [4];  [1]
  1. Section of Oral Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (Japan)
  2. Section of Pharmacology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (Japan)
  3. Section of Diagnostic Oral Pathology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (Japan)
  4. Section of Maxillofacial Surgery, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (Japan)
Publication Date:
OSTI Identifier:
22210168
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 424; Journal Issue: 3; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIBODIES; BONE MARROW CELLS; CARBON 13; CARBON 17; CARCINOMAS; GENES; MICE; SKELETON

Citation Formats

Oue, Erika, Section of Maxillofacial Surgery, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Global Center of Excellence, Lee, Ji-Won, Sakamoto, Kei, Iimura, Tadahiro, Global Center of Excellence, Aoki, Kazuhiro, Kayamori, Kou, Department of Pathology, Ome Municipal General Hospital, Ome, Tokyo, Michi, Yasuyuki, Yamashiro, Masashi, Harada, Kiyoshi, Amagasa, Teruo, Yamaguchi, Akira, and Global Center of Excellence. CXCL2 synthesized by oral squamous cell carcinoma is involved in cancer-associated bone destruction. United States: N. p., 2012. Web. doi:10.1016/J.BBRC.2012.06.132.
Oue, Erika, Section of Maxillofacial Surgery, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Global Center of Excellence, Lee, Ji-Won, Sakamoto, Kei, Iimura, Tadahiro, Global Center of Excellence, Aoki, Kazuhiro, Kayamori, Kou, Department of Pathology, Ome Municipal General Hospital, Ome, Tokyo, Michi, Yasuyuki, Yamashiro, Masashi, Harada, Kiyoshi, Amagasa, Teruo, Yamaguchi, Akira, & Global Center of Excellence. CXCL2 synthesized by oral squamous cell carcinoma is involved in cancer-associated bone destruction. United States. https://doi.org/10.1016/J.BBRC.2012.06.132
Oue, Erika, Section of Maxillofacial Surgery, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Global Center of Excellence, Lee, Ji-Won, Sakamoto, Kei, Iimura, Tadahiro, Global Center of Excellence, Aoki, Kazuhiro, Kayamori, Kou, Department of Pathology, Ome Municipal General Hospital, Ome, Tokyo, Michi, Yasuyuki, Yamashiro, Masashi, Harada, Kiyoshi, Amagasa, Teruo, Yamaguchi, Akira, and Global Center of Excellence. 2012. "CXCL2 synthesized by oral squamous cell carcinoma is involved in cancer-associated bone destruction". United States. https://doi.org/10.1016/J.BBRC.2012.06.132.
@article{osti_22210168,
title = {CXCL2 synthesized by oral squamous cell carcinoma is involved in cancer-associated bone destruction},
author = {Oue, Erika and Section of Maxillofacial Surgery, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University and Global Center of Excellence and Lee, Ji-Won and Sakamoto, Kei and Iimura, Tadahiro and Global Center of Excellence and Aoki, Kazuhiro and Kayamori, Kou and Department of Pathology, Ome Municipal General Hospital, Ome, Tokyo and Michi, Yasuyuki and Yamashiro, Masashi and Harada, Kiyoshi and Amagasa, Teruo and Yamaguchi, Akira and Global Center of Excellence},
abstractNote = {Highlights: Black-Right-Pointing-Pointer Oral cancer cells synthesize CXCL2. Black-Right-Pointing-Pointer CXCL2 synthesized by oral cancer is involved in osteoclastogenesis. Black-Right-Pointing-Pointer CXCL2-neutralizing antibody inhibited osteoclastogenesis induced by oral cancer cells. Black-Right-Pointing-Pointer We first report the role of CXCL2 in cancer-associated bone destruction. -- Abstract: To explore the mechanism of bone destruction associated with oral cancer, we identified factors that stimulate osteoclastic bone resorption in oral squamous cell carcinoma. Two clonal cell lines, HSC3-C13 and HSC3-C17, were isolated from the maternal oral cancer cell line, HSC3. The conditioned medium from HSC3-C13 cells showed the highest induction of Rankl expression in the mouse stromal cell lines ST2 and UAMS-32 as compared to that in maternal HSC3 cells and HSC3-C17 cells, which showed similar activity. The conditioned medium from HSC3-C13 cells significantly increased the number of osteoclasts in a co-culture with mouse bone marrow cells and UAMS-32 cells. Xenograft tumors generated from these clonal cell lines into the periosteal region of the parietal bone in athymic mice showed that HSC3-C13 cells caused extensive bone destruction and a significant increase in osteoclast numbers as compared to HSC3-C17 cells. Gene expression was compared between HSC3-C13 and HSC3-C17 cells by using microarray analysis, which showed that CXCL2 gene was highly expressed in HSC3-C13 cells as compared to HSC3-C17 cells. Immunohistochemical staining revealed the localization of CXCL2 in human oral squamous cell carcinomas. The increase in osteoclast numbers induced by the HSC3-C13-conditioned medium was dose-dependently inhibited by addition of anti-human CXCL2-neutralizing antibody in a co-culture system. Recombinant CXCL2 increased the expression of Rankl in UAMS-32 cells. These results indicate that CXCL2 is involved in bone destruction induced by oral cancer. This is the first report showing the role of CXCL2 in cancer-associated bone destruction.},
doi = {10.1016/J.BBRC.2012.06.132},
url = {https://www.osti.gov/biblio/22210168}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 424,
place = {United States},
year = {Fri Aug 03 00:00:00 EDT 2012},
month = {Fri Aug 03 00:00:00 EDT 2012}
}