Characterization of distinct mesenchymal-like cell populations from human skeletal muscle in situ and in vitro

Journal Article · Fri Sep 10 00:00:00 EDT 2010 · Experimental Cell Research

DOI:https://doi.org/10.1016/J.YEXCR.2010.04.020· OSTI ID:22209901

Lecourt, Severine ^[1]; Marolleau, Jean-Pierre ^[2]; Fromigue, Olivia ^[3]; Vauchez, Karine ^[1]; Andriamanalijaona, Rina ^[4]; Ternaux, Brigitte ^[2]; Lacassagne, Marie-Noelle ^[2]; Robert, Isabelle ^[2]; Boumediene, Karim ^[4]; Chereau, Frederic ^[5]; Marie, Pierre ^[3]; others, and

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1. UPMC/AIM UMR S 974, Groupe Hospitalier Pitie-Salpetriere, Paris (France)
2. Laboratoire de Therapie Cellulaire, Hopital Saint Louis, Paris (France)
3. INSERM U606, Universite Paris 07, Hopital Lariboisiere, Paris (France)
4. Laboratoire de Biochimie des Tissus Conjonctifs, Faculte de Medecine, Caen (France)
5. Myosix S.A., Saint-Germain en Laye (France)

Human skeletal muscle is an essential source of various cellular progenitors with potential therapeutic perspectives. We first used extracellular markers to identify in situ the main cell types located in a satellite position or in the endomysium of the skeletal muscle. Immunohistology revealed labeling of cells by markers of mesenchymal (CD13, CD29, CD44, CD47, CD49, CD62, CD73, CD90, CD105, CD146, and CD15 in this study), myogenic (CD56), angiogenic (CD31, CD34, CD106, CD146), hematopoietic (CD10, CD15, CD34) lineages. We then analysed cell phenotypes and fates in short- and long-term cultures of dissociated muscle biopsies in a proliferation medium favouring the expansion of myogenic cells. While CD56{sup +} cells grew rapidly, a population of CD15{sup +} cells emerged, partly from CD56{sup +} cells, and became individualized. Both populations expressed mesenchymal markers similar to that harboured by human bone marrow-derived mesenchymal stem cells. In differentiation media, both CD56{sup +} and CD15{sup +} cells shared osteogenic and chondrogenic abilities, while CD56{sup +} cells presented a myogenic capacity and CD15{sup +} cells presented an adipogenic capacity. An important proportion of cells expressed the CD34 antigen in situ and immediately after muscle dissociation. However, CD34 antigen did not persist in culture and this initial population gave rise to adipogenic cells. These results underline the diversity of human muscle cells, and the shared or restricted commitment abilities of the main lineages under defined conditions.

• OSTI ID: 22209901
• Journal Information: Experimental Cell Research, Vol. 316, Issue 15; Other Information: Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ACTIN
ALKALINE PHOSPHATASE
ANTIGENS
BIOPSY
BONE MARROW
CATTLE
IN VITRO
LABELLING
MONOCLONAL ANTIBODIES
MUSCLES
PHENOTYPE
SKELETON
STEM CELLS