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Title: The critical role of myostatin in differentiation of sheep myoblasts

Abstract

Highlights: Black-Right-Pointing-Pointer Identification of the effective and specific shRNA to knockdown MSTN. Black-Right-Pointing-Pointer Overexpression of MSTN reversibly suppressed myogenic differentiation. Black-Right-Pointing-Pointer shRNA knockdown of endogenous MSTN promoted ovine myoblast differentiation. Black-Right-Pointing-Pointer MSTN inhibits myogenic differentiation through down-regulation of MyoD and Myogenin and up-regulation of Smad3. Black-Right-Pointing-Pointer Provides a promise for the generation of transgenic sheep to improve meat productivity. -- Abstract: Myostatin [MSTN, also known as growth differentiation factor 8 (GDF8)], is an inhibitor of skeletal muscle growth. Blockade of MSTN function has been reported to result in increased muscle mass in mice. However, its role in myoblast differentiation in farm animals has not been determined. In the present study, we sought to determine the role of MSTN in the differentiation of primary sheep myoblasts. We found that ectopic overexpression of MSTN resulted in lower fusion index in sheep myoblasts, which indicated the repression of myoblast differentiation. This phenotypic change was reversed by shRNA knockdown of the ectopically expressed MSTN in the cells. In contrast, shRNA knockdown of the endogenous MSTN resulted in induction of myogenic differentiation. Additional studies revealed that the induction of differentiation by knocking down the ectopically or endogenously expressed MSTN was accompanied by up-regulation of MyoDmore » and myogenin, and down-regulation of Smad3. Our results demonstrate that MSTN plays critical role in myoblast differentiation in sheep, analogous to that in mice. This study also suggests that shRNA knockdown of MSTN could be a potentially promising approach to improve sheep muscle growth, so as to increase meat productivity.« less

Authors:
 [1]; ; ; ; ;  [2];  [3];  [2]
  1. College of Life Science and Technology, Xinjiang University, Urumqi (China)
  2. Xinjiang Laboratory of Animal Biotechnology, Urumqi (China)
  3. The State Engineering Laboratory of AIDS Vaccine, College of Life Science, Jilin University, Changchun (China)
Publication Date:
OSTI Identifier:
22207878
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 422; Journal Issue: 3; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL GROWTH; GENE REGULATION; MEAT; MICE; MYOBLASTS; PRODUCTIVITY; SHEEP

Citation Formats

Liu, Chenxi, Xinjiang Laboratory of Animal Biotechnology, Urumqi, Li, Wenrong, Zhang, Xuemei, Zhang, Ning, He, Sangang, Huang, Juncheng, Laboratory of Grass-fed Animal Genetics, Breeding and Reproduction of Ministry of Agriculture, Urumqi, Animal Biotechnological Research Center, Xinjiang Academy of Animal Science, Urumqi, Ge, Yubin, Liu, Mingjun, Laboratory of Grass-fed Animal Genetics, Breeding and Reproduction of Ministry of Agriculture, Urumqi, and Animal Biotechnological Research Center, Xinjiang Academy of Animal Science, Urumqi. The critical role of myostatin in differentiation of sheep myoblasts. United States: N. p., 2012. Web. doi:10.1016/J.BBRC.2012.04.151.
Liu, Chenxi, Xinjiang Laboratory of Animal Biotechnology, Urumqi, Li, Wenrong, Zhang, Xuemei, Zhang, Ning, He, Sangang, Huang, Juncheng, Laboratory of Grass-fed Animal Genetics, Breeding and Reproduction of Ministry of Agriculture, Urumqi, Animal Biotechnological Research Center, Xinjiang Academy of Animal Science, Urumqi, Ge, Yubin, Liu, Mingjun, Laboratory of Grass-fed Animal Genetics, Breeding and Reproduction of Ministry of Agriculture, Urumqi, & Animal Biotechnological Research Center, Xinjiang Academy of Animal Science, Urumqi. The critical role of myostatin in differentiation of sheep myoblasts. United States. https://doi.org/10.1016/J.BBRC.2012.04.151
Liu, Chenxi, Xinjiang Laboratory of Animal Biotechnology, Urumqi, Li, Wenrong, Zhang, Xuemei, Zhang, Ning, He, Sangang, Huang, Juncheng, Laboratory of Grass-fed Animal Genetics, Breeding and Reproduction of Ministry of Agriculture, Urumqi, Animal Biotechnological Research Center, Xinjiang Academy of Animal Science, Urumqi, Ge, Yubin, Liu, Mingjun, Laboratory of Grass-fed Animal Genetics, Breeding and Reproduction of Ministry of Agriculture, Urumqi, and Animal Biotechnological Research Center, Xinjiang Academy of Animal Science, Urumqi. 2012. "The critical role of myostatin in differentiation of sheep myoblasts". United States. https://doi.org/10.1016/J.BBRC.2012.04.151.
@article{osti_22207878,
title = {The critical role of myostatin in differentiation of sheep myoblasts},
author = {Liu, Chenxi and Xinjiang Laboratory of Animal Biotechnology, Urumqi and Li, Wenrong and Zhang, Xuemei and Zhang, Ning and He, Sangang and Huang, Juncheng and Laboratory of Grass-fed Animal Genetics, Breeding and Reproduction of Ministry of Agriculture, Urumqi and Animal Biotechnological Research Center, Xinjiang Academy of Animal Science, Urumqi and Ge, Yubin and Liu, Mingjun and Laboratory of Grass-fed Animal Genetics, Breeding and Reproduction of Ministry of Agriculture, Urumqi and Animal Biotechnological Research Center, Xinjiang Academy of Animal Science, Urumqi},
abstractNote = {Highlights: Black-Right-Pointing-Pointer Identification of the effective and specific shRNA to knockdown MSTN. Black-Right-Pointing-Pointer Overexpression of MSTN reversibly suppressed myogenic differentiation. Black-Right-Pointing-Pointer shRNA knockdown of endogenous MSTN promoted ovine myoblast differentiation. Black-Right-Pointing-Pointer MSTN inhibits myogenic differentiation through down-regulation of MyoD and Myogenin and up-regulation of Smad3. Black-Right-Pointing-Pointer Provides a promise for the generation of transgenic sheep to improve meat productivity. -- Abstract: Myostatin [MSTN, also known as growth differentiation factor 8 (GDF8)], is an inhibitor of skeletal muscle growth. Blockade of MSTN function has been reported to result in increased muscle mass in mice. However, its role in myoblast differentiation in farm animals has not been determined. In the present study, we sought to determine the role of MSTN in the differentiation of primary sheep myoblasts. We found that ectopic overexpression of MSTN resulted in lower fusion index in sheep myoblasts, which indicated the repression of myoblast differentiation. This phenotypic change was reversed by shRNA knockdown of the ectopically expressed MSTN in the cells. In contrast, shRNA knockdown of the endogenous MSTN resulted in induction of myogenic differentiation. Additional studies revealed that the induction of differentiation by knocking down the ectopically or endogenously expressed MSTN was accompanied by up-regulation of MyoD and myogenin, and down-regulation of Smad3. Our results demonstrate that MSTN plays critical role in myoblast differentiation in sheep, analogous to that in mice. This study also suggests that shRNA knockdown of MSTN could be a potentially promising approach to improve sheep muscle growth, so as to increase meat productivity.},
doi = {10.1016/J.BBRC.2012.04.151},
url = {https://www.osti.gov/biblio/22207878}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 422,
place = {United States},
year = {Fri Jun 08 00:00:00 EDT 2012},
month = {Fri Jun 08 00:00:00 EDT 2012}
}