Low-intensity pulsed ultrasound regulates proliferation and differentiation of osteoblasts through osteocytes

Journal Article · Fri Feb 10 00:00:00 EST 2012 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2012.01.014· OSTI ID:22207695

Li, Lei ^[1]; Yang, Zheng ^[1]; Zhang, Hai ^[2]; Chen, Wenchuan ^[1]; Chen, Mengshi ^[3]; Zhu, Zhimin ^[1]

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1. State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu (China)
2. Department of Restorative Dentistry, School of Dentistry, University of Washington, Seattle, WA (United States)
3. Department of Biomechanics, Sichuan University, Chengdu (China)

Highlights: Black-Right-Pointing-Pointer CM from LIPUS-stimulated osteocytes inhibits proliferation of osteoblasts. Black-Right-Pointing-Pointer CM from LIPUS-stimulated osteocytes enhances differentiation of osteoblasts. Black-Right-Pointing-Pointer LIPUS stimulates MLO-Y4 cells to secrete PGE{sub 2} and NO. -- Abstract: Low-intensity pulsed ultrasound (LIPUS) has been used as a safe and effective modality to enhance fracture healing. As the most abundant cells in bone, osteocytes orchestrate biological activities of effector cells via direct cell-to-cell contacts and by soluble factors. In this study, we have used the osteocytic MLO-Y4 cells to study the effects of conditioned medium from LIPUS-stimulated MLO-Y4 cells on proliferation and differentiation of osteoblastic MC3T3-E1 cells. Conditioned media from LIPUS-stimulated MLO-Y4 cells (LIPUS-Osteocyte-CM) were collected and added on MC3T3-E1 cell cultures. MC3T3-E1 cells cultured in LIPUS-Osteocyte-CM demonstrated a significant inhibition of proliferation and an increased alkaline phosphatase activity. The results of PGE{sub 2} and NO assay showed that LIPUS could enhance PGE{sub 2} and NO secretion from MLO-Y4 cells at all time points within 24 h after LIPUS stimulation. We conclude that LIPUS regulates proliferation and differentiation of osteoblasts through osteocytes in vitro. Increased secretion of PGE{sub 2} from osteocytes may play a role in this effect.

• OSTI ID: 22207695
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 418, Issue 2; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ALKALINE PHOSPHATASE
BONE CELLS
CELL CULTURES
CELL PROLIFERATION
FRACTURES
HEALING
IN VITRO
INHIBITION
NITRIC OXIDE
PROSTAGLANDINS
SECRETION
SKELETON
STIMULATION