The protective effect of magnesium lithospermate B against glucose-induced intracellular oxidative damage

Qu, Jian; Ren, Xian; Hou, Rui-ying; Dai, Xing-ping; Zhao, Ying-chun; Xu, Xiao-jing; Zhang, Wei; Zhou, Gan; Zhou, Hong-hao; Liu, Zhao-qian

doi:10.1016/J.BBRC.2011.06.071

Title: The protective effect of magnesium lithospermate B against glucose-induced intracellular oxidative damage

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Abstract

Highlights: {yields} LAB reduced the ROS production in HEK293T cells cultured under oxidative stress. High dose of glucose enhanced the expression of HO-1 mRNA and HO-1 protein in a time-dependent manner. {yields} LAB enhanced the expression of HO-1 mRNA and HO-1 protein in a dose-dependent manner treated with high dose of glucose. {yields} LAB plays an important role against glucose-induced intracellular oxidative damage. {yields} The enhanced expression of HO-1 mRNA and HO-1 protein caused by LAB is regulated via Nrf2 signal pathway. -- Abstract: Objectives: To investigate the effects of magnesium lithospermate B (LAB) on intracellular reactive oxygen species (ROS) production induced by high dose of glucose or H{sub 2}O{sub 2}, we explored the influences of LAB on the expression of heme oxygenase-1 (HO-1) and nuclear factor E2-related factor-2 (Nrf2) in HEK293T cells after treatment with high dose of glucose. Materials and methods: The total nuclear proteins in HEK293T cells were extracted with Cytoplasmic Protein Extraction Kit. The ROS level was determined by flow cytometry. The mRNA and protein expression of HO-1 and Nrf2 were determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot. Results: LAB reduced the ROS production in HEK293T cells cultured under oxidative stress.more »« less

Authors:

Qu, Jian ^[1]; Ren, Xian ^[2]; Hou, Rui-ying; Dai, Xing-ping ^[1]; Zhao, Ying-chun ^[3]; Xu, Xiao-jing; Zhang, Wei; Zhou, Gan; Zhou, Hong-hao ^[1]; Liu, Zhao-qian ^[1]

Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, Central South University, Xiangya School of Medicine, Changsha 410078 (China)
Shanghai Green Valley Pharmaceutical Co., Ltd., Shanghai 201304 (China)
Laboratories of Functional Genomics and Proteomics, Creighton University Medical Center, Omaha, NE 68131 (United States)

Publication Date:: Fri Jul 22 00:00:00 EDT 2011

OSTI Identifier:: 22207407

Resource Type:: Journal Article

Journal Name:: Biochemical and Biophysical Research Communications

Additional Journal Information:: Journal Volume: 411; Journal Issue: 1; Other Information: Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; CATS; CREATINE; DOSES; ECR HEATING; GLUCOSE; GLUTATHIONE; HEME; HYDROGEN PEROXIDE; MAGNESIUM; MESSENGER-RNA; OXIDATION; PEROXIDASES; POLYMERASE CHAIN REACTION; SUPEROXIDE DISMUTASE; TIME DEPENDENCE; TRANSFERASES

Citation Formats


                    Qu, Jian, Ren, Xian, Hou, Rui-ying, Dai, Xing-ping, Zhao, Ying-chun, Xu, Xiao-jing, Zhang, Wei, Zhou, Gan, Zhou, Hong-hao, and Liu, Zhao-qian. The protective effect of magnesium lithospermate B against glucose-induced intracellular oxidative damage.  United States: N. p., 2011. 
        Web.  doi:10.1016/J.BBRC.2011.06.071.

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                    Qu, Jian, Ren, Xian, Hou, Rui-ying, Dai, Xing-ping, Zhao, Ying-chun, Xu, Xiao-jing, Zhang, Wei, Zhou, Gan, Zhou, Hong-hao, & Liu, Zhao-qian. The protective effect of magnesium lithospermate B against glucose-induced intracellular oxidative damage.  United States.  https://doi.org/10.1016/J.BBRC.2011.06.071

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                    Qu, Jian, Ren, Xian, Hou, Rui-ying, Dai, Xing-ping, Zhao, Ying-chun, Xu, Xiao-jing, Zhang, Wei, Zhou, Gan, Zhou, Hong-hao, and Liu, Zhao-qian. 2011.  
        "The protective effect of magnesium lithospermate B against glucose-induced intracellular oxidative damage".  United States.  https://doi.org/10.1016/J.BBRC.2011.06.071.

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@article{osti_22207407,

  title        = {The protective effect of magnesium lithospermate B against glucose-induced intracellular oxidative damage},

  author       = {Qu, Jian and Ren, Xian and Hou, Rui-ying and Dai, Xing-ping and Zhao, Ying-chun and Xu, Xiao-jing and Zhang, Wei and Zhou, Gan and Zhou, Hong-hao and Liu, Zhao-qian},

  abstractNote = {Highlights: {yields} LAB reduced the ROS production in HEK293T cells cultured under oxidative stress. High dose of glucose enhanced the expression of HO-1 mRNA and HO-1 protein in a time-dependent manner. {yields} LAB enhanced the expression of HO-1 mRNA and HO-1 protein in a dose-dependent manner treated with high dose of glucose. {yields} LAB plays an important role against glucose-induced intracellular oxidative damage. {yields} The enhanced expression of HO-1 mRNA and HO-1 protein caused by LAB is regulated via Nrf2 signal pathway. -- Abstract: Objectives: To investigate the effects of magnesium lithospermate B (LAB) on intracellular reactive oxygen species (ROS) production induced by high dose of glucose or H{sub 2}O{sub 2}, we explored the influences of LAB on the expression of heme oxygenase-1 (HO-1) and nuclear factor E2-related factor-2 (Nrf2) in HEK293T cells after treatment with high dose of glucose. Materials and methods: The total nuclear proteins in HEK293T cells were extracted with Cytoplasmic Protein Extraction Kit. The ROS level was determined by flow cytometry. The mRNA and protein expression of HO-1 and Nrf2 were determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot. Results: LAB reduced the ROS production in HEK293T cells cultured under oxidative stress. High dose of glucose enhanced the expression of HO-1 mRNA and HO-1 protein in a time-dependent manner. LAB enhanced the expression of HO-1 mRNA and HO-1 protein in a dose-dependent manner treated with high dose of glucose. The amount of Nrf2 translocation was enhanced after cells were pretreated with 50 {mu}mol/L or 100 {mu}mol/L LAB. Silencing of Nrf2 gene eliminated the enhanced expression of HO-1 protein induced by high dose of glucose plus LAB. Conclusions: LAB plays an important role against glucose-induced intracellular oxidative damage. The enhanced expression of HO-1 mRNA and HO-1 protein caused by LAB is regulated via Nrf2 signal pathway.},

  doi          = {10.1016/J.BBRC.2011.06.071},

  url          = {https://www.osti.gov/biblio/22207407},
  journal      = {Biochemical and Biophysical Research Communications},

  issn         = {0006-291X},

  number       = 1,

  volume       = 411,

  place        = {United States},

  year         = {Fri Jul 22 00:00:00 EDT 2011},

  month        = {Fri Jul 22 00:00:00 EDT 2011}

}

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