Physical properties of mesenchymal stem cells are coordinated by the perinuclear actin cap
- Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531 (Japan)
- Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-46 Nakoji, Amagasaki, Hyogo 661-0974 (Japan)
Highlights: {yields} Cell thickness and stiffness of rat MSC are inversely correlated. {yields} Perinuclear actin cap coordinates the cell thickness and stiffness of rat MSC. {yields} Physical properties of rat MSCs regulate their proliferation activity. {yields} Physical properties of MSCs are potent indicators for their physiological functions. -- Abstract: Mesenchymal stem cells (MSCs) have been extensively investigated for their applications in regenerative medicine. Successful use of MSCs in cell-based therapies will rely on the ability to effectively identify their properties and functions with a relatively non-destructive methodology. In this study, we measured the surface stiffness and thickness of rat MSCs with atomic force microscopy and clarified their relation at a single-cell level. The role of the perinuclear actin cap in regulating the thickness, stiffness, and proliferative activity of these cells was also determined by using several actin cytoskeleton-modifying reagents. This study has helped elucidate a possible link between the physical properties and the physiological function of the MSCs, and the corresponding regulatory role of the actin cytoskeleton.
- OSTI ID:
- 22204930
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 409, Issue 1; Other Information: Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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