RNF43 interacts with NEDL1 and regulates p53-mediated transcription

Journal Article · Fri Jan 07 00:00:00 EST 2011 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2010.11.082· OSTI ID:22204729

Shinada, Keisuke ^[1]; Tsukiyama, Tadasuke ^[1]; Sho, Takuya ^[1]; Okumura, Fumihiko ^[1]; Asaka, Masahiro ^[2]; Hatakeyama, Shigetsugu ^[1]

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1. Department of Biochemistry, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido 060-8638 (Japan)
2. Department of Gastroenterology and Hematology, Hokkaido University Graduate School of Medicine, Sapporo 060-8638 (Japan)

Research highlights: {yields} RNF43 binds to NEDD-4-like ubiquitin-protein ligase-1 (NEDL1). {yields} RNF43 interacts with p53 and suppresses transcriptional activity of p53. {yields} RNF43 attenuates apoptosis induced by ultraviolet irradiation. {yields} RNF43 is likely associated with p53-mediated apoptosis in collaboration with NEDL1 in colorectal carcinogenesis. -- Abstract: The ubiquitin-proteasomal system plays a crucial role in oncogenesis in colorectal tissues. Recent studies have shown that stability of {beta}-catenin, which functions as an oncogene for colorectal cancer, is regulated by ubiquitin-mediated degradation. It has been reported that a putative E3 ubiquitin ligase, RNF43, is highly expressed in human colorectal carcinoma and that RNF43 promotes cell growth. However, the involvement of RNF43 in carcinogenesis has not been fully elucidated. In this study, we found by using yeast two-hybrid screening that RNF43 binds to NEDD-4-like ubiquitin-protein ligase-1 (NEDL1), which enhances pro-apoptotic activity by p53. In addition, we found that RNF43 also interacts with p53 and that RNF43 suppresses transcriptional activity of p53 in H1299 cells and attenuates apoptosis induced by ultraviolet irradiation. These findings suggest that RNF43 is associated with p53-mediated apoptosis in collaboration with NEDL1 in colorectal carcinogenesis.

• OSTI ID: 22204729
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 404, Issue 1; Other Information: Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS
ANIMAL TISSUES
APOPTOSIS
BIOLOGICAL RADIATION EFFECTS
CARCINOGENESIS
CARCINOMAS
HYBRIDIZATION
IRRADIATION
LIGASES
ONCOGENES
TRANSCRIPTION
ULTRAVIOLET RADIATION
YEASTS