AHNAK1 and AHNAK2 are costameric proteins: AHNAK1 affects transverse skeletal muscle fiber stiffness
- Max-Delbrueck-Centrum fuer Molekulare Medizin, D-13092 Berlin (Germany)
- JPK Instruments AG, Bouchestrasse 12, D-12435 Berlin (Germany)
- School of Medicine, Keio University, 35 Shinanomachi, Shinjuku, Tokyo 160-8582 (Japan)
Research highlights: {yields} AHNAK1 and AHNAK2 are costameric proteins. {yields} Intact membrane repair in AHNAK1-deficient mice. {yields} AHNAK1{sup -/-} single fibers have a higher transverse stiffness. -- Abstract: The AHNAK scaffold PDZ-protein family is implicated in various cellular processes including membrane repair; however, AHNAK function and subcellular localization in skeletal muscle are unclear. We used specific AHNAK1 and AHNAK2 antibodies to analyzed the detailed localization of both proteins in mouse skeletal muscle. Co-localization of AHNAK1 and AHNAK2 with vinculin clearly demonstrates that both proteins are components of the costameric network. In contrast, no AHNAK expression was detected in the T-tubule system. A laser wounding assay with AHNAK1-deficient fibers suggests that AHNAK1 is not involved in membrane repair. Using atomic force microscopy (AFM), we observed a significantly higher transverse stiffness of AHNAK1{sup -/-} fibers. These findings suggest novel functions of AHNAK proteins in skeletal muscle.
- OSTI ID:
- 22202807
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 401, Issue 1; Other Information: Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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