{beta}-Catenin/LEF1 activated enamelin expression in ameloblast-like cells
Abstract
Research highlights: {yields} {beta}-Catenin/LEF1 complex could activate enamelin gene transcription. {yields} {beta}-Catenin/LEF1 can directly bind to enamelin 5' regulatory region. {yields} Wnt/{beta}-catenin signaling can upregulate enamelin expression in ameloblast-like cells. -- Abstract: Enamelin is an ameloblast-specific matrix protein believed to play essential roles in enamel formation. However, mechanisms of enamelin transcription regulation are not clear. {beta}-Catenin/LEF1 is a key transcriptional complex involved in tooth development. In this study, the role of {beta}-catenin/LEF1 in enamelin expression was investigated. The 5'-flanking region of the mouse enamelin gene was analyzed and cloned. Co-transfection analysis and mutation assays revealed that two conserved LEF1 responsive elements located at -1002 and -597 bp upstream of the enamelin translation initiation site could augment transcriptional activity of the enamelin. The interaction between the enamelin elements and {beta}-catenin/LEF1 was further confirmed by electrophoresis mobility shift assays and chromatin immunoprecipitation assays. In addition, LiCl treatment induced nuclear translocation of {beta}-catenin and elevated endogenous enamelin expression in mouse ameloblast-like cells. The results suggested that Wnt/{beta}-catenin signaling could function in enamelin gene expression by direct interaction through two conserved LEF1 responsive elements on the enamelin gene in ameloblast-like cells.
- Authors:
-
- Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology, Beijing 100081 (China)
- Department of Biochemistry and Molecular Biology, Peking University School of Basic Medical Sciences, Beijing 100191 (China)
- Publication Date:
- OSTI Identifier:
- 22202717
- Resource Type:
- Journal Article
- Journal Name:
- Biochemical and Biophysical Research Communications
- Additional Journal Information:
- Journal Volume: 398; Journal Issue: 3; Other Information: Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; CHROMATIN; ELECTROPHORESIS; ENAMELS; GENE REGULATION; GENES; LITHIUM CHLORIDES; MICE; MUTATIONS; PROTEINS; TEETH; TRANSCRIPTION; TRANSLOCATION
Citation Formats
Tian, Hua, Lv, Ping, Ma, Kangtao, Zhou, Chunyan, and Gao, Xuejun. {beta}-Catenin/LEF1 activated enamelin expression in ameloblast-like cells. United States: N. p., 2010.
Web. doi:10.1016/J.BBRC.2010.06.111.
Tian, Hua, Lv, Ping, Ma, Kangtao, Zhou, Chunyan, & Gao, Xuejun. {beta}-Catenin/LEF1 activated enamelin expression in ameloblast-like cells. United States. https://doi.org/10.1016/J.BBRC.2010.06.111
Tian, Hua, Lv, Ping, Ma, Kangtao, Zhou, Chunyan, and Gao, Xuejun. 2010.
"{beta}-Catenin/LEF1 activated enamelin expression in ameloblast-like cells". United States. https://doi.org/10.1016/J.BBRC.2010.06.111.
@article{osti_22202717,
title = {{beta}-Catenin/LEF1 activated enamelin expression in ameloblast-like cells},
author = {Tian, Hua and Lv, Ping and Ma, Kangtao and Zhou, Chunyan and Gao, Xuejun},
abstractNote = {Research highlights: {yields} {beta}-Catenin/LEF1 complex could activate enamelin gene transcription. {yields} {beta}-Catenin/LEF1 can directly bind to enamelin 5' regulatory region. {yields} Wnt/{beta}-catenin signaling can upregulate enamelin expression in ameloblast-like cells. -- Abstract: Enamelin is an ameloblast-specific matrix protein believed to play essential roles in enamel formation. However, mechanisms of enamelin transcription regulation are not clear. {beta}-Catenin/LEF1 is a key transcriptional complex involved in tooth development. In this study, the role of {beta}-catenin/LEF1 in enamelin expression was investigated. The 5'-flanking region of the mouse enamelin gene was analyzed and cloned. Co-transfection analysis and mutation assays revealed that two conserved LEF1 responsive elements located at -1002 and -597 bp upstream of the enamelin translation initiation site could augment transcriptional activity of the enamelin. The interaction between the enamelin elements and {beta}-catenin/LEF1 was further confirmed by electrophoresis mobility shift assays and chromatin immunoprecipitation assays. In addition, LiCl treatment induced nuclear translocation of {beta}-catenin and elevated endogenous enamelin expression in mouse ameloblast-like cells. The results suggested that Wnt/{beta}-catenin signaling could function in enamelin gene expression by direct interaction through two conserved LEF1 responsive elements on the enamelin gene in ameloblast-like cells.},
doi = {10.1016/J.BBRC.2010.06.111},
url = {https://www.osti.gov/biblio/22202717},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 398,
place = {United States},
year = {Fri Jul 30 00:00:00 EDT 2010},
month = {Fri Jul 30 00:00:00 EDT 2010}
}