skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: {beta}-Catenin/LEF1 activated enamelin expression in ameloblast-like cells

Abstract

Research highlights: {yields} {beta}-Catenin/LEF1 complex could activate enamelin gene transcription. {yields} {beta}-Catenin/LEF1 can directly bind to enamelin 5' regulatory region. {yields} Wnt/{beta}-catenin signaling can upregulate enamelin expression in ameloblast-like cells. -- Abstract: Enamelin is an ameloblast-specific matrix protein believed to play essential roles in enamel formation. However, mechanisms of enamelin transcription regulation are not clear. {beta}-Catenin/LEF1 is a key transcriptional complex involved in tooth development. In this study, the role of {beta}-catenin/LEF1 in enamelin expression was investigated. The 5'-flanking region of the mouse enamelin gene was analyzed and cloned. Co-transfection analysis and mutation assays revealed that two conserved LEF1 responsive elements located at -1002 and -597 bp upstream of the enamelin translation initiation site could augment transcriptional activity of the enamelin. The interaction between the enamelin elements and {beta}-catenin/LEF1 was further confirmed by electrophoresis mobility shift assays and chromatin immunoprecipitation assays. In addition, LiCl treatment induced nuclear translocation of {beta}-catenin and elevated endogenous enamelin expression in mouse ameloblast-like cells. The results suggested that Wnt/{beta}-catenin signaling could function in enamelin gene expression by direct interaction through two conserved LEF1 responsive elements on the enamelin gene in ameloblast-like cells.

Authors:
;  [1];  [2];  [2];  [1]
  1. Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology, Beijing 100081 (China)
  2. Department of Biochemistry and Molecular Biology, Peking University School of Basic Medical Sciences, Beijing 100191 (China)
Publication Date:
OSTI Identifier:
22202717
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 398; Journal Issue: 3; Other Information: Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CHROMATIN; ELECTROPHORESIS; ENAMELS; GENE REGULATION; GENES; LITHIUM CHLORIDES; MICE; MUTATIONS; PROTEINS; TEETH; TRANSCRIPTION; TRANSLOCATION

Citation Formats

Tian, Hua, Lv, Ping, Ma, Kangtao, Zhou, Chunyan, and Gao, Xuejun. {beta}-Catenin/LEF1 activated enamelin expression in ameloblast-like cells. United States: N. p., 2010. Web. doi:10.1016/J.BBRC.2010.06.111.
Tian, Hua, Lv, Ping, Ma, Kangtao, Zhou, Chunyan, & Gao, Xuejun. {beta}-Catenin/LEF1 activated enamelin expression in ameloblast-like cells. United States. https://doi.org/10.1016/J.BBRC.2010.06.111
Tian, Hua, Lv, Ping, Ma, Kangtao, Zhou, Chunyan, and Gao, Xuejun. 2010. "{beta}-Catenin/LEF1 activated enamelin expression in ameloblast-like cells". United States. https://doi.org/10.1016/J.BBRC.2010.06.111.
@article{osti_22202717,
title = {{beta}-Catenin/LEF1 activated enamelin expression in ameloblast-like cells},
author = {Tian, Hua and Lv, Ping and Ma, Kangtao and Zhou, Chunyan and Gao, Xuejun},
abstractNote = {Research highlights: {yields} {beta}-Catenin/LEF1 complex could activate enamelin gene transcription. {yields} {beta}-Catenin/LEF1 can directly bind to enamelin 5' regulatory region. {yields} Wnt/{beta}-catenin signaling can upregulate enamelin expression in ameloblast-like cells. -- Abstract: Enamelin is an ameloblast-specific matrix protein believed to play essential roles in enamel formation. However, mechanisms of enamelin transcription regulation are not clear. {beta}-Catenin/LEF1 is a key transcriptional complex involved in tooth development. In this study, the role of {beta}-catenin/LEF1 in enamelin expression was investigated. The 5'-flanking region of the mouse enamelin gene was analyzed and cloned. Co-transfection analysis and mutation assays revealed that two conserved LEF1 responsive elements located at -1002 and -597 bp upstream of the enamelin translation initiation site could augment transcriptional activity of the enamelin. The interaction between the enamelin elements and {beta}-catenin/LEF1 was further confirmed by electrophoresis mobility shift assays and chromatin immunoprecipitation assays. In addition, LiCl treatment induced nuclear translocation of {beta}-catenin and elevated endogenous enamelin expression in mouse ameloblast-like cells. The results suggested that Wnt/{beta}-catenin signaling could function in enamelin gene expression by direct interaction through two conserved LEF1 responsive elements on the enamelin gene in ameloblast-like cells.},
doi = {10.1016/J.BBRC.2010.06.111},
url = {https://www.osti.gov/biblio/22202717}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 398,
place = {United States},
year = {Fri Jul 30 00:00:00 EDT 2010},
month = {Fri Jul 30 00:00:00 EDT 2010}
}