A poly(dimethylsiloxane)-based device enabling time-lapse imaging with high spatial resolution

Journal Article · Fri Feb 12 00:00:00 EST 2010 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2009.12.181· OSTI ID:22202367

Hirano, Masahiko ^[1]; Hoshida, Tetsushi ^[1]; Sakaue-Sawano, Asako ^[1]; Miyawaki, Atsushi ^[1]

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1. ERATO Life Function Dynamics Project, Japan Science and Technology Agency, 2-1 Hirosawa, Wako, Saitama 351-0198 (Japan)

We have developed a regulator-free device that enables long-term incubation of mammalian cells for epi-fluorescence imaging, based on a concept that the size of sample to be gassed and heated is reduced to observation scale. A poly(dimethylsiloxane) block stamped on a coverslip works as a long-lasting supplier of CO{sub 2}-rich gas to adjust bicarbonate-containing medium in a tiny chamber at physiological pH, and an oil-immersion objective warms cells across the coverslip. A time-lapse imaging experiment using HeLa cells stably expressing fluorescent cell-cycle indicators showed that the cells in the chamber proliferated with normal cell-cycle period over 2 days.

• OSTI ID: 22202367
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 392, Issue 3; Other Information: Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ACID CARBONATES
APERTURES
CARBON DIOXIDE
CATTLE
CELL CYCLE
FLUORESCENCE
HELA CELLS
PH VALUE
RESPIRATION
SPATIAL RESOLUTION