SUMOylated IRF-1 shows oncogenic potential by mimicking IRF-2

Journal Article · Fri Jan 01 00:00:00 EST 2010 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2009.11.166· OSTI ID:22199994

Park, Sun-Mi ^[1]; Chae, Myounghee ^[2]; Kim, Bo-Kyoung ^[1]; Seo, Taegun ^[3]; Jang, Ik-Soon; Choi, Jong-Soon ^[4]; Kim, Il-Chul ^[5]; Lee, Je-Ho ^[6]; Park, Junsoo ^[1]

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1. Yonsei University, Division of Biological Science and Technology, Wonju 220-100 (Korea, Republic of)
2. Clinical Trial Center for Functional Foods, Chonbuk National University Hospital, Jeonju 561-712 (Korea, Republic of)
3. Department of Life Science, Dongguk Univ-Seoul, Seoul 100-715 (Korea, Republic of)
4. Proteome Research Team, Korea Basic Science Institute, Daejeon 305-333 (Korea, Republic of)
5. School of Biological Sciences and Biotechnology, Chonnam National University, Gwangju 500-757 (Korea, Republic of)
6. Molecular Therapy Research Center, Sungkyunkwan University, Seoul 135-710 (Korea, Republic of)

Interferon regulatory factor-1 (IRF-1) is an interferon-induced transcriptional activator that suppresses tumors by impeding cell proliferation. Recently, we demonstrated that the level of SUMOylated IRF-1 is elevated in tumor cells, and that SUMOylation of IRF-1 attenuates its tumor-suppressive function. Here we report that SUMOylated IRF-1 mimics IRF-2, an antagonistic repressor, and shows oncogenic potential. To demonstrate the role of SUMOylated IRF-1 in tumorigenesis, we used SUMO-IRF-1 recombinant protein. Stable expression of SUMO-IRF-1 in NIH3T3 cells resulted in focus formation and anchorage-independent growth in soft agar. Inoculation of SUMO-IRF-1-transfected cells into athymic nude mice resulted in tumor formation and infiltration of adipose tissues. Finally, we demonstrated that SUMO-IRF-1 transforms NIH3T3 cells in a dose-dependent manner suggesting that SUMOylated IRF-1 may act as an oncogenic protein in tumor cells.

• OSTI ID: 22199994
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 391, Issue 1; Other Information: Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ADIPOSE TISSUE
AGAR
CELL PROLIFERATION
DOSES
INOCULATION
INTERFERON
MICE
NEOPLASMS
TUMOR CELLS