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Title: Increased isoprostane levels in oleic acid-induced lung injury

Abstract

The present study was performed to examine a role of oxidative stress in oleic acid-induced lung injury model. Fifteen anesthetized sheep were ventilated and instrumented with a lung lymph fistula and vascular catheters for blood gas analysis and measurement of isoprostanes (8-epi prostaglandin F2{alpha}). Following stable baseline measurements, oleic acid (0.08 ml/kg) was administered and observed 4 h. Isoprostane was measured by gas chromatography mass spectrometry with the isotope dilution method. Isoprostane levels in plasma and lung lymph were significantly increased 2 h after oleic acid administration and then decreased at 4 h. The percent increases in isoprostane levels in plasma and lung lymph at 2 h were significantly correlated with deteriorated oxygenation at the same time point, respectively. These findings suggest that oxidative stress is involved in the pathogenesis of the pulmonary fat embolism-induced acute lung injury model in sheep and that the increase relates with the deteriorated oxygenation.

Authors:
 [1];  [2]; ; ;  [2];  [1];  [3]
  1. Department of Anesthesiology and Resuscitation, Shinshu University School of Medicine, Matsumoto (Japan)
  2. First Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto (Japan)
  3. Department of Molecular Cell Biology, Institute of DNA Medicine, Jikei University School of Medicine, Tokyo (Japan)
Publication Date:
OSTI Identifier:
22199839
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 388; Journal Issue: 2; Other Information: Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BLOOD; EDEMA; GAS ANALYSIS; GAS CHROMATOGRAPHY; INJURIES; LUNGS; LYMPH; MASS SPECTROSCOPY; OLEIC ACID; OXIDATION; OXIDIZERS; PATHOGENESIS; PERMEABILITY; PROSTAGLANDINS; SHEEP; STRESSES

Citation Formats

Ono, Koichi, Koizumi, Tomonobu, Tsushima, Kenji, Yoshikawa, Sumiko, Yokoyama, Toshiki, Nakagawa, Rikimaru, and Obata, Toru. Increased isoprostane levels in oleic acid-induced lung injury. United States: N. p., 2009. Web. doi:10.1016/J.BBRC.2009.07.157.
Ono, Koichi, Koizumi, Tomonobu, Tsushima, Kenji, Yoshikawa, Sumiko, Yokoyama, Toshiki, Nakagawa, Rikimaru, & Obata, Toru. Increased isoprostane levels in oleic acid-induced lung injury. United States. https://doi.org/10.1016/J.BBRC.2009.07.157
Ono, Koichi, Koizumi, Tomonobu, Tsushima, Kenji, Yoshikawa, Sumiko, Yokoyama, Toshiki, Nakagawa, Rikimaru, and Obata, Toru. 2009. "Increased isoprostane levels in oleic acid-induced lung injury". United States. https://doi.org/10.1016/J.BBRC.2009.07.157.
@article{osti_22199839,
title = {Increased isoprostane levels in oleic acid-induced lung injury},
author = {Ono, Koichi and Koizumi, Tomonobu and Tsushima, Kenji and Yoshikawa, Sumiko and Yokoyama, Toshiki and Nakagawa, Rikimaru and Obata, Toru},
abstractNote = {The present study was performed to examine a role of oxidative stress in oleic acid-induced lung injury model. Fifteen anesthetized sheep were ventilated and instrumented with a lung lymph fistula and vascular catheters for blood gas analysis and measurement of isoprostanes (8-epi prostaglandin F2{alpha}). Following stable baseline measurements, oleic acid (0.08 ml/kg) was administered and observed 4 h. Isoprostane was measured by gas chromatography mass spectrometry with the isotope dilution method. Isoprostane levels in plasma and lung lymph were significantly increased 2 h after oleic acid administration and then decreased at 4 h. The percent increases in isoprostane levels in plasma and lung lymph at 2 h were significantly correlated with deteriorated oxygenation at the same time point, respectively. These findings suggest that oxidative stress is involved in the pathogenesis of the pulmonary fat embolism-induced acute lung injury model in sheep and that the increase relates with the deteriorated oxygenation.},
doi = {10.1016/J.BBRC.2009.07.157},
url = {https://www.osti.gov/biblio/22199839}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 2,
volume = 388,
place = {United States},
year = {Fri Oct 16 00:00:00 EDT 2009},
month = {Fri Oct 16 00:00:00 EDT 2009}
}