Increased isoprostane levels in oleic acid-induced lung injury
Abstract
The present study was performed to examine a role of oxidative stress in oleic acid-induced lung injury model. Fifteen anesthetized sheep were ventilated and instrumented with a lung lymph fistula and vascular catheters for blood gas analysis and measurement of isoprostanes (8-epi prostaglandin F2{alpha}). Following stable baseline measurements, oleic acid (0.08 ml/kg) was administered and observed 4 h. Isoprostane was measured by gas chromatography mass spectrometry with the isotope dilution method. Isoprostane levels in plasma and lung lymph were significantly increased 2 h after oleic acid administration and then decreased at 4 h. The percent increases in isoprostane levels in plasma and lung lymph at 2 h were significantly correlated with deteriorated oxygenation at the same time point, respectively. These findings suggest that oxidative stress is involved in the pathogenesis of the pulmonary fat embolism-induced acute lung injury model in sheep and that the increase relates with the deteriorated oxygenation.
- Authors:
-
- Department of Anesthesiology and Resuscitation, Shinshu University School of Medicine, Matsumoto (Japan)
- First Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto (Japan)
- Department of Molecular Cell Biology, Institute of DNA Medicine, Jikei University School of Medicine, Tokyo (Japan)
- Publication Date:
- OSTI Identifier:
- 22199839
- Resource Type:
- Journal Article
- Journal Name:
- Biochemical and Biophysical Research Communications
- Additional Journal Information:
- Journal Volume: 388; Journal Issue: 2; Other Information: Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; BLOOD; EDEMA; GAS ANALYSIS; GAS CHROMATOGRAPHY; INJURIES; LUNGS; LYMPH; MASS SPECTROSCOPY; OLEIC ACID; OXIDATION; OXIDIZERS; PATHOGENESIS; PERMEABILITY; PROSTAGLANDINS; SHEEP; STRESSES
Citation Formats
Ono, Koichi, Koizumi, Tomonobu, Tsushima, Kenji, Yoshikawa, Sumiko, Yokoyama, Toshiki, Nakagawa, Rikimaru, and Obata, Toru. Increased isoprostane levels in oleic acid-induced lung injury. United States: N. p., 2009.
Web. doi:10.1016/J.BBRC.2009.07.157.
Ono, Koichi, Koizumi, Tomonobu, Tsushima, Kenji, Yoshikawa, Sumiko, Yokoyama, Toshiki, Nakagawa, Rikimaru, & Obata, Toru. Increased isoprostane levels in oleic acid-induced lung injury. United States. https://doi.org/10.1016/J.BBRC.2009.07.157
Ono, Koichi, Koizumi, Tomonobu, Tsushima, Kenji, Yoshikawa, Sumiko, Yokoyama, Toshiki, Nakagawa, Rikimaru, and Obata, Toru. 2009.
"Increased isoprostane levels in oleic acid-induced lung injury". United States. https://doi.org/10.1016/J.BBRC.2009.07.157.
@article{osti_22199839,
title = {Increased isoprostane levels in oleic acid-induced lung injury},
author = {Ono, Koichi and Koizumi, Tomonobu and Tsushima, Kenji and Yoshikawa, Sumiko and Yokoyama, Toshiki and Nakagawa, Rikimaru and Obata, Toru},
abstractNote = {The present study was performed to examine a role of oxidative stress in oleic acid-induced lung injury model. Fifteen anesthetized sheep were ventilated and instrumented with a lung lymph fistula and vascular catheters for blood gas analysis and measurement of isoprostanes (8-epi prostaglandin F2{alpha}). Following stable baseline measurements, oleic acid (0.08 ml/kg) was administered and observed 4 h. Isoprostane was measured by gas chromatography mass spectrometry with the isotope dilution method. Isoprostane levels in plasma and lung lymph were significantly increased 2 h after oleic acid administration and then decreased at 4 h. The percent increases in isoprostane levels in plasma and lung lymph at 2 h were significantly correlated with deteriorated oxygenation at the same time point, respectively. These findings suggest that oxidative stress is involved in the pathogenesis of the pulmonary fat embolism-induced acute lung injury model in sheep and that the increase relates with the deteriorated oxygenation.},
doi = {10.1016/J.BBRC.2009.07.157},
url = {https://www.osti.gov/biblio/22199839},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 2,
volume = 388,
place = {United States},
year = {Fri Oct 16 00:00:00 EDT 2009},
month = {Fri Oct 16 00:00:00 EDT 2009}
}