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Title: Important role of heparan sulfate in postnatal islet growth and insulin secretion

Abstract

Heparan sulfate (HS) binds with several signaling molecules and regulates ligand-receptor interactions, playing an essential role in embryonic development. Here we showed that HS was intensively expressed in pancreatic islet {beta}-cells after 1 week of age in mice. The enzymatic removal of HS in isolated islets resulted in attenuated glucose-induced insulin secretion with a concomitant reduction in gene expression of several key components in the insulin secretion machinery. We further depleted islet HS by inactivating the exostosin tumor-like 3 gene specifically in {beta}-cells. These mice exhibited abnormal islet morphology with reduced {beta}-cell proliferation after 1 week of age and glucose intolerance due to defective insulin secretion. These results demonstrate that islet HS is involved in the regulation of postnatal islet maturation and required to ensure normal insulin secretion.

Authors:
;  [1];  [2]; ; ;  [3];  [1]; ;  [4];  [1];  [5]; ;  [1];  [6];  [7];  [3];  [5];
  1. Department of Advanced Biological Sciences for Regeneration (Kotobiken Medical Laboratories), Tohoku University Graduate School of Medicine, Sendai 980-8575 (Japan)
  2. Department of Medical Biochemistry, Iwate Medical University School of Pharmacy, Yahaba-cho 028-3603 (Japan)
  3. Laboratory of Proteoglycan Signaling and Therapeutics, Hokkaido University Graduate School of Life Science, Sapporo 001-0021 (Japan)
  4. Division of Transgenic Animal Science, Advanced Science Research Center, Kanazawa University, Kanazawa 920-8640 (Japan)
  5. Department of Biochemistry, Nara Medical University, Kashihara 634-8521 (Japan)
  6. Department of Biochemistry, University of Toyama Graduate School of Medicine and Pharmaceutical Sciences, Toyama 930-0194 (Japan)
  7. Department of Surgery, Tohoku University Graduate School of Medicine, Sendai 980-8574 (Japan)
Publication Date:
OSTI Identifier:
22199700
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 383; Journal Issue: 1; Other Information: Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; GENES; GLUCOSE; GROWTH; INSULIN; INTERACTIONS; LIGANDS; MICE; NEOPLASMS; PANCREAS; REDUCTION; REGULATIONS; SECRETION; SULFATES

Citation Formats

Takahashi, Iwao, Noguchi, Naoya, Nata, Koji, Yamada, Shuhei, Kaneiwa, Tomoyuki, Mizumoto, Shuji, Ikeda, Takayuki, Sugihara, Kazushi, Asano, Masahide, Yoshikawa, Takeo, Yamauchi, Akiyo, Shervani, Nausheen Jamal, Uruno, Akira, Kato, Ichiro, Unno, Michiaki, Sugahara, Kazuyuki, Takasawa, Shin, and others, and. Important role of heparan sulfate in postnatal islet growth and insulin secretion. United States: N. p., 2009. Web. doi:10.1016/J.BBRC.2009.03.140.
Takahashi, Iwao, Noguchi, Naoya, Nata, Koji, Yamada, Shuhei, Kaneiwa, Tomoyuki, Mizumoto, Shuji, Ikeda, Takayuki, Sugihara, Kazushi, Asano, Masahide, Yoshikawa, Takeo, Yamauchi, Akiyo, Shervani, Nausheen Jamal, Uruno, Akira, Kato, Ichiro, Unno, Michiaki, Sugahara, Kazuyuki, Takasawa, Shin, & others, and. Important role of heparan sulfate in postnatal islet growth and insulin secretion. United States. https://doi.org/10.1016/J.BBRC.2009.03.140
Takahashi, Iwao, Noguchi, Naoya, Nata, Koji, Yamada, Shuhei, Kaneiwa, Tomoyuki, Mizumoto, Shuji, Ikeda, Takayuki, Sugihara, Kazushi, Asano, Masahide, Yoshikawa, Takeo, Yamauchi, Akiyo, Shervani, Nausheen Jamal, Uruno, Akira, Kato, Ichiro, Unno, Michiaki, Sugahara, Kazuyuki, Takasawa, Shin, and others, and. 2009. "Important role of heparan sulfate in postnatal islet growth and insulin secretion". United States. https://doi.org/10.1016/J.BBRC.2009.03.140.
@article{osti_22199700,
title = {Important role of heparan sulfate in postnatal islet growth and insulin secretion},
author = {Takahashi, Iwao and Noguchi, Naoya and Nata, Koji and Yamada, Shuhei and Kaneiwa, Tomoyuki and Mizumoto, Shuji and Ikeda, Takayuki and Sugihara, Kazushi and Asano, Masahide and Yoshikawa, Takeo and Yamauchi, Akiyo and Shervani, Nausheen Jamal and Uruno, Akira and Kato, Ichiro and Unno, Michiaki and Sugahara, Kazuyuki and Takasawa, Shin and others, and},
abstractNote = {Heparan sulfate (HS) binds with several signaling molecules and regulates ligand-receptor interactions, playing an essential role in embryonic development. Here we showed that HS was intensively expressed in pancreatic islet {beta}-cells after 1 week of age in mice. The enzymatic removal of HS in isolated islets resulted in attenuated glucose-induced insulin secretion with a concomitant reduction in gene expression of several key components in the insulin secretion machinery. We further depleted islet HS by inactivating the exostosin tumor-like 3 gene specifically in {beta}-cells. These mice exhibited abnormal islet morphology with reduced {beta}-cell proliferation after 1 week of age and glucose intolerance due to defective insulin secretion. These results demonstrate that islet HS is involved in the regulation of postnatal islet maturation and required to ensure normal insulin secretion.},
doi = {10.1016/J.BBRC.2009.03.140},
url = {https://www.osti.gov/biblio/22199700}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 383,
place = {United States},
year = {Fri May 22 00:00:00 EDT 2009},
month = {Fri May 22 00:00:00 EDT 2009}
}