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Title: HIV-1 pre-mRNA commitment to Rev mediated export through PSF and Matrin 3

Abstract

Human immunodeficiency virus gene expression and replication are regulated at several levels. Incompletely spliced viral RNAs and full-length genomic RNA contain the RRE element and are bound by the viral trans-acting protein Rev to be transported out of the nucleus. Previously we found that the nuclear matrix protein MATR3 was a cofactor of Rev-mediated RNA export. Here we show that the pleiotropic protein PSF binds viral RNA and is associated with MATR3. PSF is involved in the maintenance of a pool of RNA available for Rev activity. However, while Rev and PSF bind the viral pre-mRNA at the site of viral transcription, MATR3 interacts at a subsequent step. We propose that PSF and MATR3 define a novel pathway for RRE-containing HIV-1 RNAs that is hijacked by the viral Rev protein.

Authors:
;  [1];  [1]
  1. The Laboratory of Molecular Virology, International Centre for Genetic Engineering and Biotechnology (ICGEB), Padriciano 99, 34012 Trieste (Italy)
Publication Date:
OSTI Identifier:
22150093
Resource Type:
Journal Article
Journal Name:
Virology
Additional Journal Information:
Journal Volume: 435; Journal Issue: 2; Other Information: Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0042-6822
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AIDS VIRUS; MESSENGER-RNA; NUCLEAR MATRIX; PROTEINS; RNA PROCESSING; TRANSCRIPTION

Citation Formats

Kula, Anna, Gharu, Lavina, and Marcello, Alessandro. HIV-1 pre-mRNA commitment to Rev mediated export through PSF and Matrin 3. United States: N. p., 2013. Web. doi:10.1016/J.VIROL.2012.10.032.
Kula, Anna, Gharu, Lavina, & Marcello, Alessandro. HIV-1 pre-mRNA commitment to Rev mediated export through PSF and Matrin 3. United States. https://doi.org/10.1016/J.VIROL.2012.10.032
Kula, Anna, Gharu, Lavina, and Marcello, Alessandro. 2013. "HIV-1 pre-mRNA commitment to Rev mediated export through PSF and Matrin 3". United States. https://doi.org/10.1016/J.VIROL.2012.10.032.
@article{osti_22150093,
title = {HIV-1 pre-mRNA commitment to Rev mediated export through PSF and Matrin 3},
author = {Kula, Anna and Gharu, Lavina and Marcello, Alessandro},
abstractNote = {Human immunodeficiency virus gene expression and replication are regulated at several levels. Incompletely spliced viral RNAs and full-length genomic RNA contain the RRE element and are bound by the viral trans-acting protein Rev to be transported out of the nucleus. Previously we found that the nuclear matrix protein MATR3 was a cofactor of Rev-mediated RNA export. Here we show that the pleiotropic protein PSF binds viral RNA and is associated with MATR3. PSF is involved in the maintenance of a pool of RNA available for Rev activity. However, while Rev and PSF bind the viral pre-mRNA at the site of viral transcription, MATR3 interacts at a subsequent step. We propose that PSF and MATR3 define a novel pathway for RRE-containing HIV-1 RNAs that is hijacked by the viral Rev protein.},
doi = {10.1016/J.VIROL.2012.10.032},
url = {https://www.osti.gov/biblio/22150093}, journal = {Virology},
issn = {0042-6822},
number = 2,
volume = 435,
place = {United States},
year = {Sun Jan 20 00:00:00 EST 2013},
month = {Sun Jan 20 00:00:00 EST 2013}
}