Expression, purification, crystallization, and preliminary X-ray crystallographic analysis of OXA-17, an extended-spectrum {beta}-lactamase conferring severe antibiotic resistance

Journal Article · Mon Jul 15 00:00:00 EDT 2013 · Crystallography Reports

DOI:https://doi.org/10.1134/S1063774513040238· OSTI ID:22121687

Lee, J. H., E-mail: [e-mail]; Sohn, S. G., E-mail: [e-mail]; Jung, H. I., E-mail: [e-mail]; An, Y. J., E-mail: [e-mail]; Lee, S. H., E-mail: [e-mail] ^[1]

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1. Myongji University, Drug Resistance Proteomics Laboratory, Department of Biological Sciences (Korea, Republic of)

OXA-17, an extended-spectrum {beta}-lactamase (ESBL) conferring severe antibiotic resistance, hydrolytically inactivates {beta}-lactam antibiotics, inducing a lack of eradication of pathogenic bacteria by oxyimino {beta}-lactams and not helping hospital infection control. Thus, the enzyme is a potential target for developing antimicrobial agents against pathogens producing ESBLs. OXA-17 was purified and crystallized at 298 K. X-ray diffraction data from OXA-17 crystal have been collected to 1.85 A resolution using synchrotron radiation. The crystal of OXA-17 belongs to space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 48.37, b = 101.12, and c = 126.07 A. Analysis of the packing density shows that the asymmetric unit probably contains two molecules with a solvent content of 54.6%.

• OSTI ID: 22121687
• Journal Information: Crystallography Reports, Vol. 58, Issue 4; Other Information: Copyright (c) 2013 Pleiades Publishing, Ltd.; http://www.springer-ny.com; Country of input: International Atomic Energy Agency (IAEA); ISSN 1063-7745
• Country of Publication: United States
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
ANTIBIOTICS
ANTIMICROBIAL AGENTS
ASYMMETRY
BACTERIA
CRYSTALLIZATION
CRYSTALLOGRAPHY
CRYSTALS
ENZYMES
LACTAMS
ORTHORHOMBIC LATTICES
PATHOGENS
PURIFICATION
RESOLUTION
SOLVENTS
SPACE GROUPS
SYNCHROTRON RADIATION
TEMPERATURE RANGE 0273-0400 K
X RADIATION
X-RAY DIFFRACTION