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Title: Noncontact microsurgery of cell membranes using femtosecond laser pulses for optoinjection of specified substances into cells

Journal Article · · Quantum Electronics (Woodbury, N.Y.)

IR femtosecond laser pulses were used for microsurgery of a cell membrane aimed at local and short-duration change in its permeability and injection of specified extracellular substances into the cells. The possibility of noncontact laser delivery of the propidium iodide fluorescent dye and the pEGFP plasmid, encoding the green fluorescent protein, into the cells with preservation of the cell viability was demonstrated. (extreme light fields and their applications)

OSTI ID:
22113694
Journal Information:
Quantum Electronics (Woodbury, N.Y.), Vol. 43, Issue 4; Other Information: Country of input: International Atomic Energy Agency (IAEA); ISSN 1063-7818
Country of Publication:
United States
Language:
English