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Title: Recombinant formate dehydrogenase from Arabidopsis thaliana: Preparation, crystal growth in microgravity, and preliminary X-ray diffraction study

Abstract

Crystals of high-purity recombinant NAD{sup +}-dependent formate dehydrogenase from the higher plant Arabidopsis thaliana (AraFDH) were grown in microgravity in the Modul'-1 protein crystallization apparatus on the International Space Station. The space-grown crystals have larger sizes than those grown on Earth. X-ray diffraction data suitable for determining the three-dimensional structure were collected from the space-grown crystals to a resolution of 1.22 A using an X-ray synchrotron source. The crystals belong to sp. gr. P4{sub 3}2{sub 1}2; the unit-cell parameters are a = b = 107.865 A, c = 71.180 A, {alpha} = {beta} = {gamma} = 90 deg.

Authors:
;  [1]; ;  [2];  [3];  [1];  [2]
  1. Moscow State University, Faculty of Chemistry (Russian Federation)
  2. Russian Academy of Sciences, Shubnikov Institute of Crystallography (Russian Federation)
  3. Russian Academy of Sciences, Bach Institute of Biochemistry (Russian Federation)
Publication Date:
OSTI Identifier:
22054091
Resource Type:
Journal Article
Journal Name:
Crystallography Reports
Additional Journal Information:
Journal Volume: 55; Journal Issue: 5; Other Information: Copyright (c) 2010 Pleiades Publishing, Ltd.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 1063-7745
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; ARABIDOPSIS; CRYSTAL GROWTH; CRYSTALLIZATION; CRYSTALS; FORMATES; IMPURITIES; RESOLUTION; TETRAGONAL LATTICES; X-RAY DIFFRACTION

Citation Formats

Shabalin, I. G., E-mail: inna@ns.crys.ras.ru, Serov, A E, Skirgello, O E, Timofeev, V I, Samygina, V R, Popov, V O, Tishkov, V I, and Kuranova, I P. Recombinant formate dehydrogenase from Arabidopsis thaliana: Preparation, crystal growth in microgravity, and preliminary X-ray diffraction study. United States: N. p., 2010. Web. doi:10.1134/S1063774510050159.
Shabalin, I. G., E-mail: inna@ns.crys.ras.ru, Serov, A E, Skirgello, O E, Timofeev, V I, Samygina, V R, Popov, V O, Tishkov, V I, & Kuranova, I P. Recombinant formate dehydrogenase from Arabidopsis thaliana: Preparation, crystal growth in microgravity, and preliminary X-ray diffraction study. United States. https://doi.org/10.1134/S1063774510050159
Shabalin, I. G., E-mail: inna@ns.crys.ras.ru, Serov, A E, Skirgello, O E, Timofeev, V I, Samygina, V R, Popov, V O, Tishkov, V I, and Kuranova, I P. 2010. "Recombinant formate dehydrogenase from Arabidopsis thaliana: Preparation, crystal growth in microgravity, and preliminary X-ray diffraction study". United States. https://doi.org/10.1134/S1063774510050159.
@article{osti_22054091,
title = {Recombinant formate dehydrogenase from Arabidopsis thaliana: Preparation, crystal growth in microgravity, and preliminary X-ray diffraction study},
author = {Shabalin, I. G., E-mail: inna@ns.crys.ras.ru and Serov, A E and Skirgello, O E and Timofeev, V I and Samygina, V R and Popov, V O and Tishkov, V I and Kuranova, I P},
abstractNote = {Crystals of high-purity recombinant NAD{sup +}-dependent formate dehydrogenase from the higher plant Arabidopsis thaliana (AraFDH) were grown in microgravity in the Modul'-1 protein crystallization apparatus on the International Space Station. The space-grown crystals have larger sizes than those grown on Earth. X-ray diffraction data suitable for determining the three-dimensional structure were collected from the space-grown crystals to a resolution of 1.22 A using an X-ray synchrotron source. The crystals belong to sp. gr. P4{sub 3}2{sub 1}2; the unit-cell parameters are a = b = 107.865 A, c = 71.180 A, {alpha} = {beta} = {gamma} = 90 deg.},
doi = {10.1134/S1063774510050159},
url = {https://www.osti.gov/biblio/22054091}, journal = {Crystallography Reports},
issn = {1063-7745},
number = 5,
volume = 55,
place = {United States},
year = {Wed Sep 15 00:00:00 EDT 2010},
month = {Wed Sep 15 00:00:00 EDT 2010}
}