Recombinant formate dehydrogenase from Arabidopsis thaliana: Preparation, crystal growth in microgravity, and preliminary X-ray diffraction study
Abstract
Crystals of high-purity recombinant NAD{sup +}-dependent formate dehydrogenase from the higher plant Arabidopsis thaliana (AraFDH) were grown in microgravity in the Modul'-1 protein crystallization apparatus on the International Space Station. The space-grown crystals have larger sizes than those grown on Earth. X-ray diffraction data suitable for determining the three-dimensional structure were collected from the space-grown crystals to a resolution of 1.22 A using an X-ray synchrotron source. The crystals belong to sp. gr. P4{sub 3}2{sub 1}2; the unit-cell parameters are a = b = 107.865 A, c = 71.180 A, {alpha} = {beta} = {gamma} = 90 deg.
- Authors:
-
- Moscow State University, Faculty of Chemistry (Russian Federation)
- Russian Academy of Sciences, Shubnikov Institute of Crystallography (Russian Federation)
- Russian Academy of Sciences, Bach Institute of Biochemistry (Russian Federation)
- Publication Date:
- OSTI Identifier:
- 22054091
- Resource Type:
- Journal Article
- Journal Name:
- Crystallography Reports
- Additional Journal Information:
- Journal Volume: 55; Journal Issue: 5; Other Information: Copyright (c) 2010 Pleiades Publishing, Ltd.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 1063-7745
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; ARABIDOPSIS; CRYSTAL GROWTH; CRYSTALLIZATION; CRYSTALS; FORMATES; IMPURITIES; RESOLUTION; TETRAGONAL LATTICES; X-RAY DIFFRACTION
Citation Formats
Shabalin, I. G., E-mail: inna@ns.crys.ras.ru, Serov, A E, Skirgello, O E, Timofeev, V I, Samygina, V R, Popov, V O, Tishkov, V I, and Kuranova, I P. Recombinant formate dehydrogenase from Arabidopsis thaliana: Preparation, crystal growth in microgravity, and preliminary X-ray diffraction study. United States: N. p., 2010.
Web. doi:10.1134/S1063774510050159.
Shabalin, I. G., E-mail: inna@ns.crys.ras.ru, Serov, A E, Skirgello, O E, Timofeev, V I, Samygina, V R, Popov, V O, Tishkov, V I, & Kuranova, I P. Recombinant formate dehydrogenase from Arabidopsis thaliana: Preparation, crystal growth in microgravity, and preliminary X-ray diffraction study. United States. https://doi.org/10.1134/S1063774510050159
Shabalin, I. G., E-mail: inna@ns.crys.ras.ru, Serov, A E, Skirgello, O E, Timofeev, V I, Samygina, V R, Popov, V O, Tishkov, V I, and Kuranova, I P. 2010.
"Recombinant formate dehydrogenase from Arabidopsis thaliana: Preparation, crystal growth in microgravity, and preliminary X-ray diffraction study". United States. https://doi.org/10.1134/S1063774510050159.
@article{osti_22054091,
title = {Recombinant formate dehydrogenase from Arabidopsis thaliana: Preparation, crystal growth in microgravity, and preliminary X-ray diffraction study},
author = {Shabalin, I. G., E-mail: inna@ns.crys.ras.ru and Serov, A E and Skirgello, O E and Timofeev, V I and Samygina, V R and Popov, V O and Tishkov, V I and Kuranova, I P},
abstractNote = {Crystals of high-purity recombinant NAD{sup +}-dependent formate dehydrogenase from the higher plant Arabidopsis thaliana (AraFDH) were grown in microgravity in the Modul'-1 protein crystallization apparatus on the International Space Station. The space-grown crystals have larger sizes than those grown on Earth. X-ray diffraction data suitable for determining the three-dimensional structure were collected from the space-grown crystals to a resolution of 1.22 A using an X-ray synchrotron source. The crystals belong to sp. gr. P4{sub 3}2{sub 1}2; the unit-cell parameters are a = b = 107.865 A, c = 71.180 A, {alpha} = {beta} = {gamma} = 90 deg.},
doi = {10.1134/S1063774510050159},
url = {https://www.osti.gov/biblio/22054091},
journal = {Crystallography Reports},
issn = {1063-7745},
number = 5,
volume = 55,
place = {United States},
year = {Wed Sep 15 00:00:00 EDT 2010},
month = {Wed Sep 15 00:00:00 EDT 2010}
}
Other availability
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.