Determination of photophysical parameters of chlorophyll {alpha} in photosynthetic organisms using the method of nonlinear laser fluorimetry

Journal Article · Tue May 31 00:00:00 EDT 2011 · Quantum Electronics (Woodbury, N.Y.)

DOI:https://doi.org/10.1070/QE2011V041N05ABEH014533· OSTI ID:21552675

Gostev, T S; Fadeev, V V ^[1]

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1. Department of Physics, M.V.Lomonosov Moscow State University (Russian Federation)

We study the possibility of solving the multiparameter inverse problem of nonlinear laser fluorimetry of molecular systems with high local concentration of fluorophores (by the example of chlorophyll {alpha} molecules in photosynthetic organisms). The algorithms are proposed that allow determination of up to four photophysical parameters of chlorophyll {alpha} from the experimental fluorescence saturation curves. The uniqueness and stability of the inverse problem solution obtained using the proposed algorithms were assessed numerically. The laser spectrometer, designed in the course of carrying out the work and aimed at nonlinear laser fluorimetry in the quasi-stationary and nonstationary excitation regimes is described. The algorithms, proposed in this paper, are tested on pure cultures of microalgae Chlorella pyrenoidosa and Chlamydomonas reinhardtii under different functional conditions. (optical technologies in biophysics and medicine)

• OSTI ID: 21552675
• Journal Information: Quantum Electronics (Woodbury, N.Y.), Vol. 41, Issue 5; Other Information: DOI: 10.1070/QE2011v041n05ABEH014533; ISSN 1063-7818
• Country of Publication: United States
• Language: English

Related Subjects

74 ATOMIC AND MOLECULAR PHYSICS
60 APPLIED LIFE SCIENCES
ALGORITHMS
CHLAMYDOMONAS
CHLORELLA
CHLOROPHYLL
EXCITATION
FLUORESCENCE
FLUORESCENCE SPECTROSCOPY
LASERS
MOLECULES
NONLINEAR PROBLEMS
SPECTROMETERS
ALGAE
CARBOXYLIC ACIDS
CHLOROPHYCOTA
EMISSION
EMISSION SPECTROSCOPY
ENERGY-LEVEL TRANSITIONS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
LUMINESCENCE
MATHEMATICAL LOGIC
MEASURING INSTRUMENTS
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOTON EMISSION
PHYTOCHROMES
PIGMENTS
PLANTS
PORPHYRINS
PROTEINS
SPECTROSCOPY
UNICELLULAR ALGAE