Multimodal imaging of vascular network and blood microcirculation by optical diagnostic techniques

Journal Article · Sat Apr 30 00:00:00 EDT 2011 · Quantum Electronics (Woodbury, N.Y.)

DOI:https://doi.org/10.1070/QE2011V041N04ABEH014602· OSTI ID:21550398

Kuznetsov, Yu L; Kalchenko, V V; Meglinski, I V

We present a multimodal optical diagnostic approach for simultaneous non-invasive in vivo imaging of blood and lymphatic microvessels, utilising a combined use of fluorescence intravital microscopy and a method of dynamic light scattering. This approach makes it possible to renounce the use of fluorescent markers for visualisation of blood vessels and, therefore, significantly (tenfold) reduce the toxicity of the technique and minimise side effects caused by the use of contrast fluorescent markers. We demonstrate that along with the ability to obtain images of lymph and blood microvessels with a high spatial resolution, current multimodal approach allows one to observe in real time permeability of blood vessels. This technique appears to be promising in physiology studies of blood vessels, and especially in the study of peripheral cardiovascular system in vivo. (optical technologies in biophysics and medicine)

• OSTI ID: 21550398
• Journal Information: Quantum Electronics (Woodbury, N.Y.), Vol. 41, Issue 4; Other Information: DOI: 10.1070/QE2011v041n04ABEH014602; ISSN 1063-7818
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY
BLOOD CIRCULATION
BLOOD VESSELS
DIAGNOSTIC TECHNIQUES
FLUORESCENCE
IMAGES
IN VIVO
LIGHT SCATTERING
LYMPH
MEDICINE
MICROSCOPY
PERMEABILITY
PHYSIOLOGY
SPATIAL RESOLUTION
TOXICITY
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CARDIOVASCULAR SYSTEM
EMISSION
LUMINESCENCE
MATERIALS
ORGANS
PHOTON EMISSION
PHYSICAL PROPERTIES
RESOLUTION
SCATTERING