Efficient internalization of silica-coated iron oxide nanoparticles of different sizes by primary human macrophages and dendritic cells

Journal Article · Wed Jun 01 00:00:00 EDT 2011 · Toxicology and Applied Pharmacology

DOI:https://doi.org/10.1016/j.taap.2011.03.011· OSTI ID:21535306

Kunzmann, Andrea ^[1]; Andersson, Britta ^[2]; Vogt, Carmen ^[3]; Feliu, Neus ^[1]; Fei, Ye ^[3]; Gabrielsson, Susanne ^[2]; Toprak, Muhammet S ^[3]; Buerki-Thurnherr, Tina ^[4]; Laurent, Sophie ^[5]; Vahter, Marie ^[6]; Krug, Harald ^[4]; Muhammed, Mamoun ^[3]; Scheynius, Annika ^[2]; Fadeel, Bengt ^[1]

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1. Division of Molecular Toxicology, Institute of Environmental Medicine, Karolinska Institutet, Stockholm (Sweden)
2. Clinical Allergy Research Unit, Department of Medicine Solna, Karolinska Institutet, Stockholm (Sweden)
3. Functional Materials Division, School of Information and Communication Technology, Royal Institute of Technology, Stockholm (Sweden)
4. Laboratory for Materials, Biology Interactions, Swiss Federal Laboratories of Materials Testing and Research, St. Gallen (Switzerland)
5. NMR and Molecular Imaging Laboratory, Department of General, Organic and Biomedical Chemistry, University of Mons, Mons (Belgium)
6. Division of Metals and Health, Institute of Environmental Medicine, Karolinska Institutet, Stockholm (Sweden)

Engineered nanoparticles are being considered for a wide range of biomedical applications, from magnetic resonance imaging to 'smart' drug delivery systems. The development of novel nanomaterials for biomedical applications must be accompanied by careful scrutiny of their biocompatibility. In this regard, particular attention should be paid to the possible interactions between nanoparticles and cells of the immune system, our primary defense system against foreign invasion. On the other hand, labeling of immune cells serves as an ideal tool for visualization, diagnosis or treatment of inflammatory processes, which requires the efficient internalization of the nanoparticles into the cells of interest. Here, we compare novel monodispersed silica-coated iron oxide nanoparticles with commercially available dextran-coated iron oxide nanoparticles. The silica-coated iron oxide nanoparticles displayed excellent magnetic properties. Furthermore, they were non-toxic to primary human monocyte-derived macrophages at all doses tested whereas dose-dependent toxicity of the smaller silica-coated nanoparticles (30 nm and 50 nm) was observed for primary monocyte-derived dendritic cells, but not for the similarly small dextran-coated iron oxide nanoparticles. No macrophage or dendritic cell secretion of pro-inflammatory cytokines was observed upon administration of nanoparticles. The silica-coated iron oxide nanoparticles were taken up to a significantly higher degree when compared to the dextran-coated nanoparticles, irrespective of size. Cellular internalization of the silica-coated nanoparticles was through an active, actin cytoskeleton-dependent process. We conclude that these novel silica-coated iron oxide nanoparticles are promising materials for medical imaging, cell tracking and other biomedical applications.

• OSTI ID: 21535306
• Journal Information: Toxicology and Applied Pharmacology, Vol. 253, Issue 2; Other Information: DOI: 10.1016/j.taap.2011.03.011; PII: S0041-008X(11)00097-4; Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
DENDRITES
DEXTRAN
DIAGNOSIS
DOSES
HUMAN POPULATIONS
INFLAMMATION
IRON OXIDES
LYMPHOKINES
MACROPHAGES
MAGNETIC PROPERTIES
MICROTUBULES
MONOCYTES
NANOSTRUCTURES
NMR IMAGING
PARTICLES
SECRETION
SILICA
SUPERPARAMAGNETISM
SURFACE COATING
TOXICITY
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD SUBSTITUTES
BODY FLUIDS
CARBOHYDRATES
CELL CONSTITUENTS
CHALCOGENIDES
CONNECTIVE TISSUE CELLS
CRYSTALS
DEPOSITION
DIAGNOSTIC TECHNIQUES
DRUGS
GROWTH FACTORS
HEMATOLOGIC AGENTS
IRON COMPOUNDS
LEUKOCYTES
MAGNETISM
MATERIALS
MINERALS
MITOGENS
ORGANIC COMPOUNDS
OXIDE MINERALS
OXIDES
OXYGEN COMPOUNDS
PATHOLOGICAL CHANGES
PHAGOCYTES
PHYSICAL PROPERTIES
POLYSACCHARIDES
POPULATIONS
PROTEINS
SACCHARIDES
SOMATIC CELLS
SYMPTOMS
TRANSITION ELEMENT COMPOUNDS