Peculiarities of studying an isolated neuron by the method of laser interference microscopy

Journal Article · Sat Sep 30 00:00:00 EDT 2006 · Quantum Electronics (Woodbury, N.Y.)

DOI:https://doi.org/10.1070/QE2006V036N09ABEH013408· OSTI ID:21496539

Yusipovich, Alexander I; Kazakova, Tatiana A; Erokhova, Liudmila A; Brazhe, Nadezda A; Maksimov, Georgy V ^[1]; Novikov, Sergey M ^[2]; Lazarev, Grigory L ^[3]

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1. Department of Biology, M.V. Lomonosov Moscow State University, Moscow (Russian Federation)
2. Department of Physics, M.V. Lomonosov Moscow State University, Moscow (Russian Federation)
3. AMPHORA Laboratories, Moscow (Russian Federation)

Actual aspects of using a new method of laser interference microscopy (LIM) for studying nerve cells are discussed. The peculiarities of the LIM display of neurons are demonstrated by the example of isolated neurons of a pond snail Lymnaea stagnalis. A comparative analysis of the images of the cell and subcellular structures of a neuron obtained by the methods of interference microscopy, optical transmission microscopy, and confocal microscopy is performed. Various aspects of the application of LIM for studying the lateral dimensions and internal structure of the cytoplasm and organelles of a neuron in cytology and cell physiology are discussed. (laser biology)

• OSTI ID: 21496539
• Journal Information: Quantum Electronics (Woodbury, N.Y.), Vol. 36, Issue 9; Other Information: DOI: 10.1070/QE2006v036n09ABEH013408; ISSN 1063-7818
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CYTOLOGY
CYTOPLASM
IMAGES
INTERFERENCE
LASER RADIATION
NERVE CELLS
OPTICAL MICROSCOPY
PHYSIOLOGY
SNAILS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
BIOLOGY
CELL CONSTITUENTS
ELECTROMAGNETIC RADIATION
INVERTEBRATES
MICROSCOPY
MOLLUSCS
RADIATIONS
SOMATIC CELLS