DNA condensates organized by the capsid protein VP15 in White Spot Syndrome Virus

Jinlu, Wu; Hu, Chen; Jie, Yan

Title: DNA condensates organized by the capsid protein VP15 in White Spot Syndrome Virus

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Abstract

The White Spot Syndrome Virus (WSSV) has a large circular double-stranded DNA genome of around 300 kb and it replicates in the nucleus of the host cells. The machinery of how the viral DNA is packaged has been remained unclear. VP15, a highly basic protein, is one of the major capsid proteins found in the virus. Previously, it was shown to be a DNA binding protein and was hypothesized to participate in the viral DNA packaging process. Using Atomic Force Microscopy imaging, we show that the viral DNA is associated with a (or more) capsid proteins. The organized viral DNA qualitatively resembles the conformations of VP15 induced DNA condensates in vitro. Furthermore, single-DNA manipulation experiments revealed that VP15 is able to condense single DNA against forces of a few pico Newtons. Our results suggest that VP15 may aid in the viral DNA packaging process by directly condensing DNA.

Authors:

Jinlu, Wu ^[1]; Hu, Chen ^[2]; Jie, Yan ^[3]

Department of Biological Science, National University of Singapore, 14 Science Drive 4, Singapore 117543 (Singapore)
Mechanobiology Institute, 5A Engineering Drive 1, Singapore 117411 (Singapore)
Department of Physics, National University of Singapore, 2 Science Drive 3, Singapore 117542 (Singapore)

Publication Date:: Mon Dec 20 00:00:00 EST 2010

OSTI Identifier:: 21484506

Resource Type:: Journal Article

Journal Name:: Virology

Additional Journal Information:: Journal Volume: 408; Journal Issue: 2; Other Information: DOI: 10.1016/j.virol.2010.09.008; PII: S0042-6822(10)00583-0; Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Journal ID: ISSN 0042-6822

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; ATOMIC FORCE MICROSCOPY; CONDENSATES; DNA; PROTEINS; VIRUSES; MICROORGANISMS; MICROSCOPY; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PARASITES

Citation Formats


                    Liu Yingjie, E-mail: phyyj@nus.edu.s, Mechanobiology Institute, 5A Engineering Drive 1, Singapore 117411, Jinlu, Wu, Hu, Chen, Hew, Choy Leong, E-mail: dbshewcl@nus.edu.s, Mechanobiology Institute, 5A Engineering Drive 1, Singapore 117411, Jie, Yan, Mechanobiology Institute, 5A Engineering Drive 1, Singapore 117411, and Centre for Bioimaging Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543. DNA condensates organized by the capsid protein VP15 in White Spot Syndrome Virus.  United States: N. p., 2010. 
        Web.

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                    Liu Yingjie, E-mail: phyyj@nus.edu.s, Mechanobiology Institute, 5A Engineering Drive 1, Singapore 117411, Jinlu, Wu, Hu, Chen, Hew, Choy Leong, E-mail: dbshewcl@nus.edu.s, Mechanobiology Institute, 5A Engineering Drive 1, Singapore 117411, Jie, Yan, Mechanobiology Institute, 5A Engineering Drive 1, Singapore 117411, & Centre for Bioimaging Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543. DNA condensates organized by the capsid protein VP15 in White Spot Syndrome Virus.  United States.

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                    Liu Yingjie, E-mail: phyyj@nus.edu.s, Mechanobiology Institute, 5A Engineering Drive 1, Singapore 117411, Jinlu, Wu, Hu, Chen, Hew, Choy Leong, E-mail: dbshewcl@nus.edu.s, Mechanobiology Institute, 5A Engineering Drive 1, Singapore 117411, Jie, Yan, Mechanobiology Institute, 5A Engineering Drive 1, Singapore 117411, and Centre for Bioimaging Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543. 2010.  
        "DNA condensates organized by the capsid protein VP15 in White Spot Syndrome Virus".  United States.

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@article{osti_21484506,

  title        = {DNA condensates organized by the capsid protein VP15 in White Spot Syndrome Virus},

  author       = {Liu Yingjie, E-mail: phyyj@nus.edu.s and Mechanobiology Institute, 5A Engineering Drive 1, Singapore 117411 and Jinlu, Wu and Hu, Chen and Hew, Choy Leong, E-mail: dbshewcl@nus.edu.s and Mechanobiology Institute, 5A Engineering Drive 1, Singapore 117411 and Jie, Yan and Mechanobiology Institute, 5A Engineering Drive 1, Singapore 117411 and Centre for Bioimaging Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543},

  abstractNote = {The White Spot Syndrome Virus (WSSV) has a large circular double-stranded DNA genome of around 300 kb and it replicates in the nucleus of the host cells. The machinery of how the viral DNA is packaged has been remained unclear. VP15, a highly basic protein, is one of the major capsid proteins found in the virus. Previously, it was shown to be a DNA binding protein and was hypothesized to participate in the viral DNA packaging process. Using Atomic Force Microscopy imaging, we show that the viral DNA is associated with a (or more) capsid proteins. The organized viral DNA qualitatively resembles the conformations of VP15 induced DNA condensates in vitro. Furthermore, single-DNA manipulation experiments revealed that VP15 is able to condense single DNA against forces of a few pico Newtons. Our results suggest that VP15 may aid in the viral DNA packaging process by directly condensing DNA.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/21484506},
  journal      = {Virology},

  issn         = {0042-6822},

  number       = 2,

  volume       = 408,

  place        = {United States},

  year         = {Mon Dec 20 00:00:00 EST 2010},

  month        = {Mon Dec 20 00:00:00 EST 2010}

}

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