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Title: Structural rearrangements in the aqueous phase of cell suspensions and protein solutions induced by a light-oxygen effect

Abstract

Temperature-dependent transient processes initiated by a direct photogeneration of singlet oxygen in suspensions of human erythrocytes and solutions of serum albumin are studied. The processes appear as anomalous jumps in the temperature dependences of the deformability coefficient of erythrocytes and the refractive index of the extracellular medium and protein solution. In the temperature regions of anomalous jumps, cells and proteins transfer to a metastable state of a lower activity, but they can be isothermally photoreactivated. Simultaneously, a reversible rearrangement of the aqueous phase occurs near the cell and protein surfaces, accompanied by the formation of an extended corona (hydrogel). The transient processes are interpreted as phase transitions in the membrane of erythrocytes and conformation transitions in proteins. The interaction between erythrocytes and albumin via hydrogel is discovered (hydro-conformational interaction). A qualitative physical model of the early stages of the light-oxygen effect is proposed, in which collective magnetic interactions between the electron spins of oxygen molecules and the nuclear magnetic moments of protons in H{sub 2}O molecules play a dominant role. (special issue devoted to the 80th anniversary of academician n g basov's birth)

Authors:
; ; ; ;  [1];  [2]; ;  [3]; ; ;  [4]
  1. P.N. Lebedev Physics Institute, Russian Academy of Sciences, Moscow (Russian Federation)
  2. N.N. Blokhin Cancer Research Center, Russian Academy of Medical Sciences, Moscow (Russian Federation)
  3. Urals State Technical University, Ekaterinburg (Russian Federation)
  4. A.M. Prokhorov General Physics Institute, Russian Academy of Sciences, Moscow (Russian Federation)
Publication Date:
OSTI Identifier:
21470265
Resource Type:
Journal Article
Journal Name:
Quantum Electronics (Woodbury, N.Y.)
Additional Journal Information:
Journal Volume: 33; Journal Issue: 2; Other Information: DOI: 10.1070/QE2003v033n02ABEH002376; Journal ID: ISSN 1063-7818
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; 74 ATOMIC AND MOLECULAR PHYSICS; ALBUMINS; ELECTRONS; ERYTHROCYTES; INTERACTIONS; MOLECULES; NUCLEAR MAGNETIC MOMENTS; OXYGEN; PHASE TRANSFORMATIONS; PHOTON ACTIVATION ANALYSIS; PROTONS; REFRACTIVE INDEX; SOLUTIONS; SPIN; SURFACES; SUSPENSIONS; TEMPERATURE DEPENDENCE; VISIBLE RADIATION; WATER; ACTIVATION ANALYSIS; ANGULAR MOMENTUM; BARYONS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CHEMICAL ANALYSIS; DISPERSIONS; ELECTROMAGNETIC RADIATION; ELEMENTARY PARTICLES; ELEMENTS; FERMIONS; HADRONS; HOMOGENEOUS MIXTURES; HYDROGEN COMPOUNDS; LEPTONS; MAGNETIC MOMENTS; MATERIALS; MIXTURES; NONDESTRUCTIVE ANALYSIS; NONMETALS; NUCLEAR PROPERTIES; NUCLEONS; OPTICAL PROPERTIES; ORGANIC COMPOUNDS; OXYGEN COMPOUNDS; PARTICLE PROPERTIES; PHYSICAL PROPERTIES; PROTEINS; RADIATIONS

Citation Formats

Zakharov, S D, Nguen, K T, Panasenko, N A, Perov, S N, Timofeev, Yu P, Ivanov, Andrei V, Wolf, E B, Skopinov, S A, Danilov, V P, Murina, T M, and Novikov, E G. Structural rearrangements in the aqueous phase of cell suspensions and protein solutions induced by a light-oxygen effect. United States: N. p., 2003. Web. doi:10.1070/QE2003V033N02ABEH002376.
Zakharov, S D, Nguen, K T, Panasenko, N A, Perov, S N, Timofeev, Yu P, Ivanov, Andrei V, Wolf, E B, Skopinov, S A, Danilov, V P, Murina, T M, & Novikov, E G. Structural rearrangements in the aqueous phase of cell suspensions and protein solutions induced by a light-oxygen effect. United States. https://doi.org/10.1070/QE2003V033N02ABEH002376
Zakharov, S D, Nguen, K T, Panasenko, N A, Perov, S N, Timofeev, Yu P, Ivanov, Andrei V, Wolf, E B, Skopinov, S A, Danilov, V P, Murina, T M, and Novikov, E G. 2003. "Structural rearrangements in the aqueous phase of cell suspensions and protein solutions induced by a light-oxygen effect". United States. https://doi.org/10.1070/QE2003V033N02ABEH002376.
@article{osti_21470265,
title = {Structural rearrangements in the aqueous phase of cell suspensions and protein solutions induced by a light-oxygen effect},
author = {Zakharov, S D and Nguen, K T and Panasenko, N A and Perov, S N and Timofeev, Yu P and Ivanov, Andrei V and Wolf, E B and Skopinov, S A and Danilov, V P and Murina, T M and Novikov, E G},
abstractNote = {Temperature-dependent transient processes initiated by a direct photogeneration of singlet oxygen in suspensions of human erythrocytes and solutions of serum albumin are studied. The processes appear as anomalous jumps in the temperature dependences of the deformability coefficient of erythrocytes and the refractive index of the extracellular medium and protein solution. In the temperature regions of anomalous jumps, cells and proteins transfer to a metastable state of a lower activity, but they can be isothermally photoreactivated. Simultaneously, a reversible rearrangement of the aqueous phase occurs near the cell and protein surfaces, accompanied by the formation of an extended corona (hydrogel). The transient processes are interpreted as phase transitions in the membrane of erythrocytes and conformation transitions in proteins. The interaction between erythrocytes and albumin via hydrogel is discovered (hydro-conformational interaction). A qualitative physical model of the early stages of the light-oxygen effect is proposed, in which collective magnetic interactions between the electron spins of oxygen molecules and the nuclear magnetic moments of protons in H{sub 2}O molecules play a dominant role. (special issue devoted to the 80th anniversary of academician n g basov's birth)},
doi = {10.1070/QE2003V033N02ABEH002376},
url = {https://www.osti.gov/biblio/21470265}, journal = {Quantum Electronics (Woodbury, N.Y.)},
issn = {1063-7818},
number = 2,
volume = 33,
place = {United States},
year = {Fri Feb 28 00:00:00 EST 2003},
month = {Fri Feb 28 00:00:00 EST 2003}
}