A study of eukaryotic response mechanisms to atmospheric pressure cold plasma by using Saccharomyces cerevisiae single gene mutants

Journal Article · Mon Sep 27 00:00:00 EDT 2010 · Applied Physics Letters

DOI:https://doi.org/10.1063/1.3491180· OSTI ID:21466935

Hongqing, Feng; Ruixue, Wang ^[1]; Peng, Sun; Haiyan, Wu ^[2]; Qi, Liu; Fangting, Li ^[1]; Jing, Fang; Jue, Zhang ^[1]; Weidong, Zhu ^[3]

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1. Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871 (China)
2. College of Engineering, Peking University, Beijing 100871 (China)
3. Department of Applied Science and Technology, Saint Peter's College, New Jersey 07031 (United States)

The mechanisms of eukaryotic cell response to cold plasma are studied. A series of single gene mutants of eukaryotic model organism Saccharomyces cerevisiae are used to compare their sensitivity to plasma treatment with the wild type. We examined 12 mutants in the oxidative stress pathway and the cell cycle pathway, in which 8 are found to be hypersensitive to plasma processing. The mutated genes' roles in the two pathways are analyzed to understand the biological response mechanisms of plasma treatment. The results demonstrate that genes from both pathways are needed for the eukaryotic cells to survive the complex plasma treatment.

• OSTI ID: 21466935
• Journal Information: Applied Physics Letters, Vol. 97, Issue 13; Other Information: DOI: 10.1063/1.3491180; (c) 2010 American Institute of Physics; ISSN 0003-6951
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ATMOSPHERIC PRESSURE
BIOLOGICAL RADIATION EFFECTS
CELL CYCLE
COLD PLASMA
COMPARATIVE EVALUATIONS
GENES
IRRADIATION
MUTANTS
OXIDATION
PROCESSING
SACCHAROMYCES CEREVISIAE
SENSITIVITY
STRESSES
BIOLOGICAL EFFECTS
CHEMICAL REACTIONS
EUMYCOTA
EVALUATION
FUNGI
MICROORGANISMS
PLANTS
PLASMA
RADIATION EFFECTS
SACCHAROMYCES
YEASTS