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Title: Effect of Irradiation on Microvascular Endothelial Cells of Parotid Glands in the Miniature Pig

Abstract

Purpose: To evaluate the effect of irradiation on microvascular endothelial cells in miniature pig parotid glands. Methods and Materials: A single 25-Gy dose of irradiation (IR) was delivered to parotid glands of 6 miniature pigs. Three other animals served as non-IR controls. Local blood flow rate in glands was measured pre- and post-IR with an ultrasonic Doppler analyzer. Samples of parotid gland tissue were taken at 4 h, 24 h, 1 week, and 2 weeks after IR for microvascular density (MVD) analysis and sphingomyelinase (SMase) assay. Histopathology and immunohistochemical staining (anti-CD31 and anti-AQP1) were used to assess morphological changes. MVD was determined by calculating the number of CD31- or AQP1-stained cells per field. A terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) apoptosis assay was used to detect apoptotic cells. The activity of acid and neutral Mg{sup 2+}-dependent SMase (ASMase and NSMase, respectively) was also assayed. Results: Local parotid gland blood flow rate decreased rapidly at 4 h post-IR and remained below control levels throughout the 14-day observation period. Parotid MVD also declined from 4 to 24 hours and remained below control levels thereafter. The activity levels of ASMase and NSMase in parotid glands increased rapidly from 4 to 24more » h post-IR and then declined gradually. The frequency of detecting apoptotic nuclei in the glands followed similar kinetics. Conclusions: Single-dose IR led to a significant reduction of MVD and local blood flow rate, indicating marked damage to microvascular endothelial cells in miniature pig parotid glands. The significant and rapid increases of ASMase and NSMase activity levels may be important in this IR-induced damage.« less

Authors:
; ; ;  [1]; ;  [2];  [1]
  1. Salivary Gland Disease Center and Molecular Laboratory for Gene Therapy, Capital Medical University School of Stomatology, Beijing (China)
  2. Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, NIH, DHHS, Bethesda, Maryland (United States)
Publication Date:
OSTI Identifier:
21438022
Resource Type:
Journal Article
Journal Name:
International Journal of Radiation Oncology, Biology and Physics
Additional Journal Information:
Journal Volume: 78; Journal Issue: 3; Other Information: DOI: 10.1016/j.ijrobp.2010.05.048; PII: S0360-3016(10)00792-3; Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Journal ID: ISSN 0360-3016
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; BLOOD FLOW; ENDOTHELIUM; MORPHOLOGICAL CHANGES; RADIOTHERAPY; SALIVARY GLANDS; SWINE; ANIMAL TISSUES; ANIMALS; BODY; DOMESTIC ANIMALS; GLANDS; MAMMALS; MEDICINE; NUCLEAR MEDICINE; ORGANS; RADIOLOGY; THERAPY; VERTEBRATES

Citation Formats

Junji, Xu, Xing, Yan, Runtao, Gao, Lisha, Mao, Cotrim, Ana P, Changyu, Zheng, Chunmei, Zhang, Baum, Bruce J., E-mail: bbaum@dir.nidcr.nih.go, Wang Songlin, E-mail: slwang@ccmu.edu.c, and Department of Biochemistry and Molecular Biology, Capital Medical University School of Basic Medical Sciences, Beijing. Effect of Irradiation on Microvascular Endothelial Cells of Parotid Glands in the Miniature Pig. United States: N. p., 2010. Web.
Junji, Xu, Xing, Yan, Runtao, Gao, Lisha, Mao, Cotrim, Ana P, Changyu, Zheng, Chunmei, Zhang, Baum, Bruce J., E-mail: bbaum@dir.nidcr.nih.go, Wang Songlin, E-mail: slwang@ccmu.edu.c, & Department of Biochemistry and Molecular Biology, Capital Medical University School of Basic Medical Sciences, Beijing. Effect of Irradiation on Microvascular Endothelial Cells of Parotid Glands in the Miniature Pig. United States.
Junji, Xu, Xing, Yan, Runtao, Gao, Lisha, Mao, Cotrim, Ana P, Changyu, Zheng, Chunmei, Zhang, Baum, Bruce J., E-mail: bbaum@dir.nidcr.nih.go, Wang Songlin, E-mail: slwang@ccmu.edu.c, and Department of Biochemistry and Molecular Biology, Capital Medical University School of Basic Medical Sciences, Beijing. 2010. "Effect of Irradiation on Microvascular Endothelial Cells of Parotid Glands in the Miniature Pig". United States.
@article{osti_21438022,
title = {Effect of Irradiation on Microvascular Endothelial Cells of Parotid Glands in the Miniature Pig},
author = {Junji, Xu and Xing, Yan and Runtao, Gao and Lisha, Mao and Cotrim, Ana P and Changyu, Zheng and Chunmei, Zhang and Baum, Bruce J., E-mail: bbaum@dir.nidcr.nih.go and Wang Songlin, E-mail: slwang@ccmu.edu.c and Department of Biochemistry and Molecular Biology, Capital Medical University School of Basic Medical Sciences, Beijing},
abstractNote = {Purpose: To evaluate the effect of irradiation on microvascular endothelial cells in miniature pig parotid glands. Methods and Materials: A single 25-Gy dose of irradiation (IR) was delivered to parotid glands of 6 miniature pigs. Three other animals served as non-IR controls. Local blood flow rate in glands was measured pre- and post-IR with an ultrasonic Doppler analyzer. Samples of parotid gland tissue were taken at 4 h, 24 h, 1 week, and 2 weeks after IR for microvascular density (MVD) analysis and sphingomyelinase (SMase) assay. Histopathology and immunohistochemical staining (anti-CD31 and anti-AQP1) were used to assess morphological changes. MVD was determined by calculating the number of CD31- or AQP1-stained cells per field. A terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) apoptosis assay was used to detect apoptotic cells. The activity of acid and neutral Mg{sup 2+}-dependent SMase (ASMase and NSMase, respectively) was also assayed. Results: Local parotid gland blood flow rate decreased rapidly at 4 h post-IR and remained below control levels throughout the 14-day observation period. Parotid MVD also declined from 4 to 24 hours and remained below control levels thereafter. The activity levels of ASMase and NSMase in parotid glands increased rapidly from 4 to 24 h post-IR and then declined gradually. The frequency of detecting apoptotic nuclei in the glands followed similar kinetics. Conclusions: Single-dose IR led to a significant reduction of MVD and local blood flow rate, indicating marked damage to microvascular endothelial cells in miniature pig parotid glands. The significant and rapid increases of ASMase and NSMase activity levels may be important in this IR-induced damage.},
doi = {},
url = {https://www.osti.gov/biblio/21438022}, journal = {International Journal of Radiation Oncology, Biology and Physics},
issn = {0360-3016},
number = 3,
volume = 78,
place = {United States},
year = {Mon Nov 01 00:00:00 EDT 2010},
month = {Mon Nov 01 00:00:00 EDT 2010}
}