One-Micron Beams for Macromolecular Crystallography at GM/CA-CAT

Journal Article · Wed Jun 23 00:00:00 EDT 2010 · AIP Conference Proceedings

DOI:https://doi.org/10.1063/1.3463229· OSTI ID:21431068

Yoder, D W; Sanishvili, R; Xu, S; Makarov, O; Benn, R; Corcoran, S; Fischetti, R F ^[1]; Vogt, S ^[2]

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1. GMCA-CAT, Biosciences Division, Argonne National Laboratory, Argonne, IL 60439 (United States)
2. X-ray Science Division, Advanced Photon Source, Argonne National Laboratory, Argonne, IL 60439 (United States)

GM/CA-CAT has developed a 1-{mu}m beam for challenging micro-diffraction experiments with macromolecular crystals (e.g. small crystals) and for radiation damage studies. Reflective (Kirkpatrick-Baez mirrors) and diffractive (Fresnel zone plates) optics have been used to focus the beam. Both cases are constrained by the need to maintain a small beam convergence. Using two different zone plates, 1.0x1.0 and 0.8x0.9 {mu}m{sup 2} (VxH,FWHM) beams were created at 15.2 keV and 18.5 keV, respectively. Additionally, by introducing a vertical focusing mirror upstream of the zone plate, a line focus at 15.2 keV was created (28x1.4 {mu}m{sup 2} VxH,FWHM) with the line oriented perpendicular to the X-ray polarization and the crystal rotation axis. Crystal-mounting stages with nanometer resolution have been assembled to profile these beams and to perform diffraction experiments.

• OSTI ID: 21431068
• Journal Information: AIP Conference Proceedings, Vol. 1234, Issue 1; Conference: SRI 2009: 10. international conference on radiation instrumentation, Melbourne (Australia), 27 Sep - 2 Oct 2009; Other Information: DOI: 10.1063/1.3463229; (c) 2010 American Institute of Physics; ISSN 0094-243X
• Country of Publication: United States
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
BEAMS
CONVERGENCE
CRYSTALLOGRAPHY
CRYSTALS
DIFFRACTION
KEV RANGE
MIRRORS
POLARIZATION
RADIATION EFFECTS
REFLECTIVITY
RESOLUTION
X RADIATION
COHERENT SCATTERING
ELECTROMAGNETIC RADIATION
ENERGY RANGE
IONIZING RADIATIONS
OPTICAL PROPERTIES
PHYSICAL PROPERTIES
RADIATIONS
SCATTERING
SURFACE PROPERTIES