Cisplatin-Conjugated Porous Gelatin Particles: Assessment of Optimal Conditions for Binding and Release

Journal Article · Sun Aug 15 00:00:00 EDT 2010 · Cardiovascular and Interventional Radiology

DOI:https://doi.org/10.1007/S00270-010-9912-0· OSTI ID:21428939

Ohta, Shinichi; Nitta, Norihisa; Sonoda, Akinaga; Nitta-Seko, Ayumi; Tanaka, Toyohiko; Takahashi, Masashi; Murata, Kiyoshi ^[1]

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1. Shiga University of Medical Science, Department of Radiology (Japan)

This study was designed to evaluate the optimal conditions for binding cisplatin and porous gelatin particles (PGPs) and to establish in vivo drug release pharmacokinetics. PGPs were immersed in cisplatin solutions under different conditions: concentration, immersion time, and temperature. Thereafter, PGPs were washed in distilled water to remove uncombined cisplatin and were then freeze-dried. The platinum concentration (PC) in the PGPs was then measured. For the in vivo release test, 50 mg/kg of the cisplatin-conjugated PGPs was implanted subcutaneously in the abdominal region of two rabbits. PCs in the blood were measured at different time intervals. PCs significantly increased in direct proportion to the concentration and immersion time (p < 0.01). Although PC increased at higher solution temperature, it was not a linear progression. For the in vivo release test, platinum was released from cisplatin-conjugated PGPs after 1 day, and the peak PC was confirmed 2 days after implantation. Platinum in the blood was detected until 7 days after implantation in one rabbit and 15 days after administration in the other rabbit. Platinum binding with PGPs increased with a higher concentration of cisplatin solution at a higher temperature over a longer duration of time. Release of cisplatin from cisplatin-conjugated PGPs was confirmed in vivo.

• OSTI ID: 21428939
• Journal Information: Cardiovascular and Interventional Radiology, Vol. 33, Issue 4; Other Information: DOI: 10.1007/s00270-010-9912-0; Copyright (c) 2010 Springer Science+Business Media, LLC and the Cardiovascular and Interventional Radiological Society of Europe (CIRSE); ISSN 0174-1551
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
ABDOMEN
ANTINEOPLASTIC DRUGS
GELATIN
IN VIVO
PLATINUM
POROUS MATERIALS
RABBITS
ANIMALS
BODY
COLLOIDS
DISPERSIONS
DRUGS
ELEMENTS
MAMMALS
MATERIALS
METALS
ORGANIC COMPOUNDS
PLATINUM METALS
PROTEINS
TRANSITION ELEMENTS
VERTEBRATES