Direct examination of cadmium bonding in rat tissues dosed with mine wastes and cadmium-containing solutions

Journal Article · Wed Jun 23 00:00:00 EDT 2010 · AIP Conference Proceedings

DOI:https://doi.org/10.1063/1.3463281· OSTI ID:21415245

Diacomanolis, V; Ng, J C ^[1]; Sadler, R ^[2]; Harris, H H ^[3]; Nomura, M ^[4]; Noller, B N ^[5]

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1. University of Queensland, National Research Centre for Environmental Toxicology, Coopers Plains, QLD, 4008 (Australia)
2. School of Public Health, Griffith University, Brisbane, QLD (Australia)
3. School of Chemistry and Physics, University of Adelaide, Adelaide, SA (Australia)
4. Photon Factory, Institute of Material Structure Science, Tsukuba (Japan)
5. University of Queensland, Centre For Mined Land Rehabilitation, St Lucia 4072 QLD (Australia)

Direct examination by XANES and EXAFS of metal bonding in tissue can be demonstrated by examining cadmium uptake and bonding in animal tissue maintained at cryogenic temperatures. XANES at the K-edge of cadmium were collected at the Photon Factory Advanced Ring (PF-AR), NW10A beam line at KEK-Tsukuba-Japan. Rats fed with 1g mine waste containing 8-400 mg/kg cadmium per 200g body weight (b.w.) or dosed by oral gavage with either cadmium chloride solution alone (at 6 mg/kg b.w.) or in combination with other salts (As, Cu or Zn), 5 days/week for 6 weeks, had 0.1-7.5 and 8-86 mg/kg cadmium in the liver or kidney, respectively. Rats given intraperitoneally (ip) or intravenously (iv) 1-4 times with 1 mg/kg b.w. cadmium solution had 30-120 mg/kg cadmium in the liver or kidney. Tissues from rats were kept and transferred at cryogenic temperature and XANES were recorded at 20 K. The spectra for rat liver samples suggested conjugation of cadmium with glutathione or association with the sulfide bond (Cd-S) of proteins and peptides. EXAFS of rat liver fed by Cd and Zn solutions showed that Cd was clearly bound to S ligands with an inter-atomic distance of 2.54 A ring for Cd-S that was similar to cadmium sulfide with an inter-atomic distance of 2.52 A ring for Cd-S. Liver or kidney of rats fed with mine wastes did not give an edge in the XANES spectra indicating little uptake of cadmium by the animals. Longer and higher dosing regimen may be required in order to observe the same Cd-S bond in the rat tissue from mine wastes, including confirmation by EXAFS.

• OSTI ID: 21415245
• Journal Information: AIP Conference Proceedings, Vol. 1234, Issue 1; Conference: SRI 2009: 10. international conference on radiation instrumentation, Melbourne (Australia), 27 Sep - 2 Oct 2009; Other Information: DOI: 10.1063/1.3463281; (c) 2010 American Institute of Physics; ISSN 0094-243X
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS
ABSORPTION SPECTROSCOPY
ANIMAL TISSUES
BEAMS
CADMIUM
CADMIUM CHLORIDES
CADMIUM SULFIDES
CRYOGENICS
FINE STRUCTURE
GLUTATHIONE
KIDNEYS
LIVER
MASS SPECTROSCOPY
MINERAL WASTES
PHOTONS
RATS
SOLUTIONS
UPTAKE
WASTE DISPOSAL
X-RAY SPECTRA
X-RAY SPECTROSCOPY
ANIMALS
BODY
BOSONS
CADMIUM COMPOUNDS
CADMIUM HALIDES
CHALCOGENIDES
CHLORIDES
CHLORINE COMPOUNDS
DIGESTIVE SYSTEM
DISPERSIONS
DRUGS
ELEMENTARY PARTICLES
ELEMENTS
GLANDS
HALIDES
HALOGEN COMPOUNDS
HOMOGENEOUS MIXTURES
INORGANIC PHOSPHORS
MAMMALS
MANAGEMENT
MASSLESS PARTICLES
METALS
MIXTURES
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PHOSPHORS
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
RESPONSE MODIFYING FACTORS
RODENTS
SOLID WASTES
SPECTRA
SPECTROSCOPY
SULFIDES
SULFUR COMPOUNDS
VERTEBRATES
WASTE MANAGEMENT
WASTES