Sialylation of Integrin beta1 is Involved in Radiation-Induced Adhesion and Migration in Human Colon Cancer Cells

Journal Article · Thu Apr 15 00:00:00 EDT 2010 · International Journal of Radiation Oncology, Biology and Physics

DOI:https://doi.org/10.1016/j.ijrobp.2009.11.022· OSTI ID:21372223

Lee, Minyoung; Lee, Hae-June ^[1]; Seo, Woo Duck ^[2]; Park, Ki Hun ^[3]; Lee, Yun-Sil ^[1]

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1. Division of Radiation Effects, Korea Institute of Radiological and Medical Sciences, Seoul (Korea, Republic of)
2. Department of Functional Corp, NICS, RDA, Miryang (Korea, Republic of)
3. Division of Applied Life Science (BK21 program), EB-NCRC, Gyeongsang National University, Jinju (Korea, Republic of)

Purpose: Previously, we reported that radiation-induced ST6 Gal I gene expression was responsible for an increase of integrin beta1 sialylation. In this study, we have further investigated the function of radiation-mediated integrin beta1 sialylation in colon cancer cells. Methods and Materials: We performed Western blotting and lectin affinity assay to analyze the expression and level of sialylated integrin beta1. After exposure to ionizing radiation (IR), adhesion and migration of cells were measured by in vitro adhesion and migration assay. Results: IR increased sialylation of integrin beta1 responsible for its increased protein stability and adhesion and migration of colon cancer cells. However, for cells with an N-glycosylation site mutant of integrin beta1 located on the I-like domain (Mu3), these effects were dramatically inhibited. In addition, integrin beta1-mediated radioresistance was not observed in cells containing this mutant. When sialylation of integrin beta1 was targeted with a sulfonamide chalcone compound, inhibition of radiation-induced sialylation of integrin beta1 and inhibition of radiation-induced adhesion and migration occurred. Conclusion: The increase of integrin beta1 sialylation by ST6 Gal I is critically involved in radiation-mediated adhesion and migration of colon cancer cells. From these findings, integrin beta1 sialylation may be a novel target for overcoming radiation-induced survival, especially radiation-induced adhesion and migration.

• OSTI ID: 21372223
• Journal Information: International Journal of Radiation Oncology, Biology and Physics, Vol. 76, Issue 5; Other Information: DOI: 10.1016/j.ijrobp.2009.11.022; PII: S0360-3016(09)03563-9; Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; ISSN 0360-3016
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS
ADHESION
IONIZING RADIATIONS
LARGE INTESTINE
MIGRATION
RECEPTORS
TUMOR CELLS
ANIMAL CELLS
BODY
DIGESTIVE SYSTEM
GASTROINTESTINAL TRACT
INTESTINES
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIATIONS