Vesicular stomatitis virus infects resident cells of the central nervous system and induces replication-dependent inflammatory responses

Journal Article · Mon May 10 00:00:00 EDT 2010 · Virology

DOI:https://doi.org/10.1016/j.virol.2010.01.025· OSTI ID:21357612

Chauhan, Vinita S; Furr, Samantha R; Sterka, David G; Nelson, Daniel A; Moerdyk-Schauwecker, Megan; Marriott, Ian ^[1]

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1. Department of Biology, 9201 University City Boulevard, University of North Carolina at Charlotte, Charlotte, NC 28223 (United States)

Vesicular stomatitis virus (VSV) infection of mice via intranasal administration results in a severe encephalitis with rapid activation and proliferation of microglia and astrocytes. We have recently shown that these glial cells express RIG-I and MDA5, cytosolic pattern recognition receptors for viral RNA. However, it is unclear whether VSV can replicate in glial cells or if such replication is required for their inflammatory responses. Here we demonstrate that primary microglia and astrocytes are permissive for VSV infection and limited productive replication. Importantly, we show that viral replication is required for robust inflammatory mediator production by these cells. Finally, we have confirmed that in vivo VSV administration can result in viral infection of glial cells in situ. These results suggest that viral replication within resident glial cells might play an important role in CNS inflammation following infection with VSV and possibly other neurotropic nonsegmented negative-strand RNA viruses.

• OSTI ID: 21357612
• Journal Information: Virology, Vol. 400, Issue 2; Other Information: DOI: 10.1016/j.virol.2010.01.025; PII: S0042-6822(10)00055-3; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CENTRAL NERVOUS SYSTEM
ENCEPHALITIS
INFLAMMATION
MICE
PATTERN RECOGNITION
PROLIFERATION
RECEPTORS
RNA
VIRUSES
ANIMALS
DISEASES
MAMMALS
MEMBRANE PROTEINS
MICROORGANISMS
NERVOUS SYSTEM
NERVOUS SYSTEM DISEASES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PATHOLOGICAL CHANGES
PROTEINS
RODENTS
SYMPTOMS
VERTEBRATES