The conserved baculovirus protein p33 (Ac92) is a flavin adenine dinucleotide-linked sulfhydryl oxidase

Journal Article · Fri Jun 05 00:00:00 EDT 2009 · Virology

DOI:https://doi.org/10.1016/j.virol.2009.04.006· OSTI ID:21357517

Long, C M ^[1]; Rohrmann, G F ^[2]

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1. Department of Biochemistry and Biophysics, Oregon State University, Corvallis, OR 97331 (United States)
2. Department of Microbiology, Oregon State University, Corvallis, OR 97331 (United States)

Open reading frame 92 of the Autographa californica baculovirus (Ac92) is one of about 30 core genes present in all sequenced baculovirus genomes. Computer analyses predicted that the Ac92 encoded protein (called p33) and several of its baculovirus orthologs were related to a family of flavin adenine dinucleotide (FAD)-linked sulfhydryl oxidases. Alignment of these proteins indicated that, although they were highly diverse, a number of amino acids in common with the Erv1p/Alrp family of sulfhydryl oxidases are present. Some of these conserved amino acids are predicted to stack against the isoalloxazine and adenine components of FAD, whereas others are involved in electron transfer. To investigate this relationship, Ac92 was expressed in bacteria as a His-tagged fusion protein, purified, and characterized both spectrophotometrically and for its enzymatic activity. The purified protein was found to have the color (yellow) and absorption spectrum consistent with it being a FAD-containing protein. Furthermore, it was demonstrated to have sulfhydryl oxidase activity using dithiothreitol and thioredoxin as substrates.

• OSTI ID: 21357517
• Journal Information: Virology, Vol. 388, Issue 2; Other Information: DOI: 10.1016/j.virol.2009.04.006; PII: S0042-6822(09)00241-4; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ABSORPTION SPECTRA
ADENINES
ALIGNMENT
AMINO ACIDS
BACTERIA
ELECTRON TRANSFER
GENES
OXIDASES
VIRUSES
AMINES
ANTIMETABOLITES
AROMATICS
AZAARENES
CARBOXYLIC ACIDS
DRUGS
ENZYMES
HETEROCYCLIC COMPOUNDS
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PARASITES
PROTEINS
PURINES
SPECTRA