Lettuce infectious yellows virus-encoded P26 induces plasmalemma deposit cytopathology
- Department of Plant Pathology, University of California, Davis, One Shields Ave., Davis, CA 95616 (United States)
- Department Produccio Vegetal i Ciencia Vegetal, Universitat de Lleida. Av. A. Rovira Roure, 191, 25198 Lleida (Spain)
- USDA-ARS, Department of Plant Pathology, University of California, One Shields, Ave., Davis, CA 95616 (United States)
Lettuce infectious yellows virus (LIYV) encodes a 26 kDa protein (P26) previously shown to associate with plasmalemma deposits (PLDs), unique LIYV-induced cytopathologies located at the plasmalemma over plasmodesmata pit fields in companion cells and phloem parenchyma. To further characterize the relationship of P26 and PLDs, we assessed localization and cytopathology induction of P26 expressed from either LIYV or a heterologous Tobacco mosaic virus (TMV) vector using green fluorescent protein (GFP) fusions, immunofluorescence microscopy, biochemical fractionation, and transmission electron microscopy (TEM). TEM analyses demonstrated that P26 not only associated with, but induced formation of PLDs in the absence of other LIYV proteins. Interestingly, PLDs induced by P26-expressing TMV were no longer confined to phloem cells. Putative P26 orthologs from two other members of the genus Crinivirus which do not induce conspicuous PLDs exhibited fractionation properties similar to LIYV P26 but were not associated with any PLD-like cytopathology.
- OSTI ID:
- 21357516
- Journal Information:
- Virology, Vol. 388, Issue 1; Other Information: DOI: 10.1016/j.virol.2009.03.016; PII: S0042-6822(09)00200-1; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; ISSN 0042-6822
- Country of Publication:
- United States
- Language:
- English
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