Cinnamaldehyde impairs high glucose-induced hypertrophy in renal interstitial fibroblasts

Journal Article · Thu Apr 15 00:00:00 EDT 2010 · Toxicology and Applied Pharmacology

DOI:https://doi.org/10.1016/j.taap.2009.12.030· OSTI ID:21344918

Chao, Louis Kuoping ^[1]; Chang, W -T; Shih, Y -W ^[2]

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1. Department of Cosmeceutics, China Medical University, Taichung 404, Taiwan (China)
2. Department of Biological Science and Technology, Chung Hwa University of Medical Technology, Tainan 717, Taiwan (China)

Cinnamaldehyde is a major and a bioactive compound isolated from the leaves of Cinnamomum osmophloeum kaneh. To explore whether cinnamaldehyde was linked to altered high glucose (HG)-mediated renal tubulointerstitial fibrosis in diabetic nephropathy (DN), the molecular mechanisms of cinnamaldehyde responsible for inhibition of HG-induced hypertrophy in renal interstitial fibroblasts were examined. We found that cinnamaldehyde caused inhibition of HG-induced cellular mitogenesis rather than cell death by either necrosis or apoptosis. There were no changes in caspase 3 activity, cleaved poly(ADP-ribose) polymerase (PARP) protein expression, and mitochondrial cytochrome c release in HG or cinnamaldehyde treatments in these cells. HG-induced extracellular signal-regulated kinase (ERK)/c-Jun N-terminal kinase (JNK)/p38 mitogen-activated protein kinase (MAPK) (but not the Janus kinase 2/signal transducers and activators of transcription) activation was markedly blocked by cinnamaldehyde. The ability of cinnamaldehyde to inhibit HG-induced hypertrophy was verified by the observation that it significantly decreased cell size, cellular hypertrophy index, and protein levels of collagen IV, fibronectin, and alpha-smooth muscle actin (alpha-SMA). The results obtained in this study suggest that cinnamaldehyde treatment of renal interstitial fibroblasts that have been stimulated by HG reduces their ability to proliferate and hypertrophy through mechanisms that may be dependent on inactivation of the ERK/JNK/p38 MAPK pathway.

• OSTI ID: 21344918
• Journal Information: Toxicology and Applied Pharmacology, Vol. 244, Issue 2; Other Information: DOI: 10.1016/j.taap.2009.12.030; PII: S0041-008X(09)00537-7; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ACTIN
ADP
APOPTOSIS
COLLAGEN
FIBROBLASTS
FIBROSIS
GLUCOSE
HYPERTROPHY
INTERSTITIALS
KIDNEYS
MITOCHONDRIA
MUSCLES
NECROSIS
RIBOSE
TRANSCRIPTION
ALDEHYDES
ANIMAL CELLS
BODY
CARBOHYDRATES
CELL CONSTITUENTS
CONNECTIVE TISSUE CELLS
CRYSTAL DEFECTS
CRYSTAL STRUCTURE
HEXOSES
MONOSACCHARIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PENTOSES
POINT DEFECTS
PROTEINS
SACCHARIDES
SCLEROPROTEINS
SOMATIC CELLS