Use of human bronchial epithelial cells (BEAS-2B) to study immunological markers resulting from exposure to PM{sub 2.5} organic extract from Puerto Rico

Journal Article · Mon Mar 15 00:00:00 EDT 2010 · Toxicology and Applied Pharmacology

DOI:https://doi.org/10.1016/j.taap.2009.12.009· OSTI ID:21344896

Fuentes-Mattei, Enrique ^[1]; Rivera, Evasomary ^[2]; Gioda, Adriana ^[1]; Sanchez-Rivera, Diana; Roman-Velazquez, Felix R ^[3]

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1. Department of Biochemistry, School of Medicine, Medical Sciences Campus, University of Puerto Rico, San Juan (Puerto Rico)
2. Department of Biology, Rio Piedras Campus, University of Puerto Rico, San Juan (Puerto Rico)
3. Department of Chemistry, Mayaguez Campus, University of Puerto Rico, Mayaguez (Puerto Rico)

Fine particulate air pollutants, mainly their organic fraction, have been demonstrated to be associated with cardiovascular and respiratory health problems. Puerto Rico has been reported to have the highest prevalence of pulmonary diseases (e.g., asthma) in the United States. The aim of this study was to assess, for the first time, the immunological response of human bronchial epithelial cells (BEAS-2B) to organic extracts isolated from airborne particulate matter (PM{sub 2.5}) in Puerto Rico. Organic extracts from PM{sub 2.5} collected throughout an 8-month period (2000-2001) were pooled (composite) in order to perform chemical analysis and biological activity testing. BEAS-2B cells were exposed to PM{sub 2.5} organic extract to assess cytotoxicity, levels of cytokines and relative gene expression of MHC-II, hPXR and CYP3A5. Our findings show that organic PM{sub 2.5} consist of toxic as well as bioactive components that can regulate the secretion of cytokines in BEAS-2B, which could modulate inflammatory response in the lung. Trace element analyses confirmed the presence of metals in organic extracts highlighting the relative high abundance of Cu and Zn in polar organic extracts. Polar organic extracts exhibited dose-dependant toxicity and were found to significantly induce the release of interleukin 6 (IL-6), IL-1beta and IL-7 while significantly inhibiting the secretion of IL-8, G-CSF and MCP-1. Moreover, MHC-II transcriptional activity was up-regulated after 24 h of exposure, whereas PXR and CYP3A5 were down-regulated. This research provides a new insight into the effects of PM{sub 2.5} organic fractions on specific effectors and their possible role in the development of respiratory inflammatory diseases in Puerto Rico.

• OSTI ID: 21344896
• Journal Information: Toxicology and Applied Pharmacology, Vol. 243, Issue 3; Other Information: DOI: 10.1016/j.taap.2009.12.009; PII: S0041-008X(09)00509-2; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
AIR POLLUTION
ASTHMA
COPPER
DOSES
GENES
INFLAMMATION
LUNGS
LYMPHOKINES
MEN
OXIDASES
TOXICITY
ANIMALS
BODY
DISEASES
ELEMENTS
ENZYMES
GROWTH FACTORS
MALES
MAMMALS
MAN
METALS
MITOGENS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PATHOLOGICAL CHANGES
POLLUTION
PRIMATES
PROTEINS
RESPIRATORY SYSTEM
RESPIRATORY SYSTEM DISEASES
SYMPTOMS
TRANSITION ELEMENTS
VERTEBRATES