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Title: Effects of adrenergic agents on the expression of zebrafish (Danio rerio) vitellogenin Ao1

Abstract

Teleost vitellogenins (VTGs) are large multidomain apolipoproteins, traditionally considered to be estrogen-responsive precursors of the major egg yolk proteins, expressed and synthesized mainly in hepatic tissue. The inducibility of VTGs has made them one of the most frequently used in vivo and in vitro biomarkers of exposure to estrogen-active substances. A significant level of zebrafish vtgAo1, a major estrogen responsive form, has been unexpectedly found in heart tissue in our present studies. Our studies on zebrafish cardiomyopathy, caused by adrenergic agonist treatment, suggest a similar protective function of the cardiac expressed vtgAo1. We hypothesize that its function is to unload surplus intracellular lipids in cardiomyocytes for 'reverse triglyceride transportation' similar to that found in lipid transport proteins in mammals. Our results also demonstrated that zebrafish vtgAo1 mRNA expression in heart can be suppressed by both {alpha}-adrenergic agonist, phenylephrine (PE) and {beta}-adrenergic agonist, isoproterenol (ISO). Furthermore, the strong stimulation of zebrafish vtgAo1 expression in plasma induced by the {beta}-adrenergic antagonist, MOXIsylyl, was detected by Enzyme-Linked ImmunoSorbent Assay (ELISA). Such stimulation cannot be suppressed by taMOXIfen, an antagonist to estrogen receptors. Thus, our present data indicate that the production of teleost VTG in vivo can be regulated not only by estrogenic agents,more » but by adrenergic signals as well.« less

Authors:
 [1]; ;  [1];  [1]
  1. Key Laboratory of Biodiversity and Conservation of Aquatic Organisms, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei 430072 (China)
Publication Date:
OSTI Identifier:
21272588
Resource Type:
Journal Article
Journal Name:
Toxicology and Applied Pharmacology
Additional Journal Information:
Journal Volume: 238; Journal Issue: 1; Other Information: DOI: 10.1016/j.taap.2009.04.004; PII: S0041-008X(09)00146-X; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0041-008X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; APOLIPOPROTEINS; BIOLOGICAL MARKERS; ENZYME IMMUNOASSAY; ESTROGENS; HEART; IN VITRO; IN VIVO; LIVER; MAMMALS; RECEPTORS; STIMULATION; TAMOXIFEN; TRIGLYCERIDES

Citation Formats

Naida, Yin, Graduate University of Chinese Academy of Sciences, Beijing 100049, Xia, Jin, Jiangyan, He, Zhan, Yin, and Graduate University of Chinese Academy of Sciences, Beijing 100049. Effects of adrenergic agents on the expression of zebrafish (Danio rerio) vitellogenin Ao1. United States: N. p., 2009. Web.
Naida, Yin, Graduate University of Chinese Academy of Sciences, Beijing 100049, Xia, Jin, Jiangyan, He, Zhan, Yin, & Graduate University of Chinese Academy of Sciences, Beijing 100049. Effects of adrenergic agents on the expression of zebrafish (Danio rerio) vitellogenin Ao1. United States.
Naida, Yin, Graduate University of Chinese Academy of Sciences, Beijing 100049, Xia, Jin, Jiangyan, He, Zhan, Yin, and Graduate University of Chinese Academy of Sciences, Beijing 100049. 2009. "Effects of adrenergic agents on the expression of zebrafish (Danio rerio) vitellogenin Ao1". United States.
@article{osti_21272588,
title = {Effects of adrenergic agents on the expression of zebrafish (Danio rerio) vitellogenin Ao1},
author = {Naida, Yin and Graduate University of Chinese Academy of Sciences, Beijing 100049 and Xia, Jin and Jiangyan, He and Zhan, Yin and Graduate University of Chinese Academy of Sciences, Beijing 100049},
abstractNote = {Teleost vitellogenins (VTGs) are large multidomain apolipoproteins, traditionally considered to be estrogen-responsive precursors of the major egg yolk proteins, expressed and synthesized mainly in hepatic tissue. The inducibility of VTGs has made them one of the most frequently used in vivo and in vitro biomarkers of exposure to estrogen-active substances. A significant level of zebrafish vtgAo1, a major estrogen responsive form, has been unexpectedly found in heart tissue in our present studies. Our studies on zebrafish cardiomyopathy, caused by adrenergic agonist treatment, suggest a similar protective function of the cardiac expressed vtgAo1. We hypothesize that its function is to unload surplus intracellular lipids in cardiomyocytes for 'reverse triglyceride transportation' similar to that found in lipid transport proteins in mammals. Our results also demonstrated that zebrafish vtgAo1 mRNA expression in heart can be suppressed by both {alpha}-adrenergic agonist, phenylephrine (PE) and {beta}-adrenergic agonist, isoproterenol (ISO). Furthermore, the strong stimulation of zebrafish vtgAo1 expression in plasma induced by the {beta}-adrenergic antagonist, MOXIsylyl, was detected by Enzyme-Linked ImmunoSorbent Assay (ELISA). Such stimulation cannot be suppressed by taMOXIfen, an antagonist to estrogen receptors. Thus, our present data indicate that the production of teleost VTG in vivo can be regulated not only by estrogenic agents, but by adrenergic signals as well.},
doi = {},
url = {https://www.osti.gov/biblio/21272588}, journal = {Toxicology and Applied Pharmacology},
issn = {0041-008X},
number = 1,
volume = 238,
place = {United States},
year = {Wed Jul 01 00:00:00 EDT 2009},
month = {Wed Jul 01 00:00:00 EDT 2009}
}