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Title: The food contaminant deoxynivalenol, decreases intestinal barrier permeability and reduces claudin expression

Abstract

'The gastrointestinal tract represents the first barrier against food contaminants as well as the first target for these toxicants. Deoxynivalenol (DON) is a mycotoxin that commonly contaminates cereals and causes various toxicological effects. Through consumption of contaminated cereals and cereal products, human and pigs are exposed to this mycotoxin. Using in vitro, ex vivo and in vivo approaches, we investigated the effects of DON on the intestinal epithelium. We demonstrated that, in intestinal epithelial cell lines from porcine (IPEC-1) or human (Caco-2) origin, DON decreases trans-epithelial electrical resistance (TEER) and increases in a time and dose-dependent manner the paracellular permeability to 4 kDa dextran and to pathogenic Escherichia coli across intestinal cell monolayers. In pig explants treated with DON, we also observed an increased permeability of intestinal tissue. These alterations of barrier function were associated with a specific reduction in the expression of claudins, which was also seen in vivo in the jejunum of piglets exposed to DON-contaminated feed. In conclusion, DON alters claudin expression and decreases the barrier function of the intestinal epithelium. Considering that high levels of DON may be present in food or feed, consumption of DON-contaminated food/feed may induce intestinal damage and has consequences for humanmore » and animal health.« less

Authors:
 [1];  [2]; ;  [1];  [1];  [3];  [4];  [5];  [6]
  1. INRA: UR66, Toulouse (France)
  2. UMR 1225, Toulouse (France)
  3. Romania
  4. UMR1054, Toulouse (France)
  5. Universidade Estadual de Londrina, Londrina (Brazil)
  6. Ecole Nationale Veterinaire, Laboratoire de Toxicologie, Toulouse (France)
Publication Date:
OSTI Identifier:
21272553
Resource Type:
Journal Article
Journal Name:
Toxicology and Applied Pharmacology
Additional Journal Information:
Journal Volume: 237; Journal Issue: 1; Other Information: DOI: 10.1016/j.taap.2009.03.003; PII: S0041-008X(09)00104-5; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0041-008X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CEREALS; DEXTRAN; ELECTRIC CONDUCTIVITY; EPITHELIUM; ESCHERICHIA COLI; FOOD; HUMAN POPULATIONS; IN VITRO; IN VIVO; PERMEABILITY; SMALL INTESTINE; SWINE

Citation Formats

Pinton, Philippe, Nougayrede, Jean-Philippe, Del Rio, Juan-Carlos, Moreno, Carolina, Marin, Daniela E, INCDBNA, Balotesti, Ferrier, Laurent, Bracarense, Ana-Paula, Kolf-Clauw, Martine, and Oswald, Isabelle P. The food contaminant deoxynivalenol, decreases intestinal barrier permeability and reduces claudin expression. United States: N. p., 2009. Web. doi:10.1016/j.taap.2009.03.003.
Pinton, Philippe, Nougayrede, Jean-Philippe, Del Rio, Juan-Carlos, Moreno, Carolina, Marin, Daniela E, INCDBNA, Balotesti, Ferrier, Laurent, Bracarense, Ana-Paula, Kolf-Clauw, Martine, & Oswald, Isabelle P. The food contaminant deoxynivalenol, decreases intestinal barrier permeability and reduces claudin expression. United States. https://doi.org/10.1016/j.taap.2009.03.003
Pinton, Philippe, Nougayrede, Jean-Philippe, Del Rio, Juan-Carlos, Moreno, Carolina, Marin, Daniela E, INCDBNA, Balotesti, Ferrier, Laurent, Bracarense, Ana-Paula, Kolf-Clauw, Martine, and Oswald, Isabelle P. 2009. "The food contaminant deoxynivalenol, decreases intestinal barrier permeability and reduces claudin expression". United States. https://doi.org/10.1016/j.taap.2009.03.003.
@article{osti_21272553,
title = {The food contaminant deoxynivalenol, decreases intestinal barrier permeability and reduces claudin expression},
author = {Pinton, Philippe and Nougayrede, Jean-Philippe and Del Rio, Juan-Carlos and Moreno, Carolina and Marin, Daniela E and INCDBNA, Balotesti and Ferrier, Laurent and Bracarense, Ana-Paula and Kolf-Clauw, Martine and Oswald, Isabelle P.},
abstractNote = {'The gastrointestinal tract represents the first barrier against food contaminants as well as the first target for these toxicants. Deoxynivalenol (DON) is a mycotoxin that commonly contaminates cereals and causes various toxicological effects. Through consumption of contaminated cereals and cereal products, human and pigs are exposed to this mycotoxin. Using in vitro, ex vivo and in vivo approaches, we investigated the effects of DON on the intestinal epithelium. We demonstrated that, in intestinal epithelial cell lines from porcine (IPEC-1) or human (Caco-2) origin, DON decreases trans-epithelial electrical resistance (TEER) and increases in a time and dose-dependent manner the paracellular permeability to 4 kDa dextran and to pathogenic Escherichia coli across intestinal cell monolayers. In pig explants treated with DON, we also observed an increased permeability of intestinal tissue. These alterations of barrier function were associated with a specific reduction in the expression of claudins, which was also seen in vivo in the jejunum of piglets exposed to DON-contaminated feed. In conclusion, DON alters claudin expression and decreases the barrier function of the intestinal epithelium. Considering that high levels of DON may be present in food or feed, consumption of DON-contaminated food/feed may induce intestinal damage and has consequences for human and animal health.},
doi = {10.1016/j.taap.2009.03.003},
url = {https://www.osti.gov/biblio/21272553}, journal = {Toxicology and Applied Pharmacology},
issn = {0041-008X},
number = 1,
volume = 237,
place = {United States},
year = {Fri May 15 00:00:00 EDT 2009},
month = {Fri May 15 00:00:00 EDT 2009}
}