Down modulation of IFN-{gamma} signaling in alveolar macrophages isolated from smokers

Journal Article · Fri May 15 00:00:00 EDT 2009 · Toxicology and Applied Pharmacology

DOI:https://doi.org/10.1016/j.taap.2009.02.021· OSTI ID:21272551

Dhillon, Navneet K ^[1]; Murphy, William J; Filla, Michael B ^[2]; Crespo, Ana J ^[3]; Latham, Heath A ^[2]; O'Brien-Ladner, Amy ^[2]

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1. Division of Pulmonary and Critical Care Medicine, Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, Kansas (United States)
2. Department of Medicine, University of Kansas Medical Center, Kansas City, Kansas (United States)
3. Institute of Molecular Pathology and Immunology of University of Porto (IPATIMUP), Porto (Portugal)

The master cytokine, IFN-{gamma} possesses a wide spectrum of biological effects and is crucial for development of the highly activated macrophage phenotype characteristically found during inflammation. However, no data exists regarding the potential influence of cigarette smoke on the status of the expression of the cell surface receptor for IFN-{gamma} (IFN-{gamma}R) on alveolar macrophages (AM) of smokers. Here in, we report reduction in the expression of the IFN-{gamma}R {alpha}-chain on AM of cigarette smokers, when compared with non-smokers. Ensuing from the loss of receptor expression on the AM of smokers there was a decrease in IFN-{gamma}-mediated cell signaling. This included a decrease in the phosphorylation of signal transducer and activator of transcription (STAT)-1 and induction of interferon regulatory factor (IRF)-1. Further, diminished activation/induction of transcription factors did not appear to result from induction of known members of the 'suppressors of cytokine signaling (SOCS)' family. Decreased IFN-{gamma} signal transduction in AM from smokers may have an important implication regarding the use of therapeutic IFN-{gamma} in the lungs of patients that develop respiratory disorders as a result of tobacco use.

• OSTI ID: 21272551
• Journal Information: Toxicology and Applied Pharmacology, Vol. 237, Issue 1; Other Information: DOI: 10.1016/j.taap.2009.02.021; PII: S0041-008X(09)00097-0; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BIOLOGICAL EFFECTS
INDUCTION
INFLAMMATION
INTERFERON
LUNGS
MACROPHAGES
MONOCYTES
NEOPLASMS
PHOSPHORYLATION
RECEPTORS
TRANSCRIPTION
TRANSCRIPTION FACTORS
TRANSDUCERS