Involvement of PKC{alpha} in PMA-induced facilitation of exocytosis and vesicle fusion in PC12 cells
- Division of Bioengineering, Nanyang Technological University, 70 Nanyang Drive, Block N1.3, Singapore 637457 (Singapore)
Phorbol-12-myristate-13-acetate, a stable analog of the important signaling membrane lipid diacylglycerol (DAG), is known to potentiate exocytosis and modulate vesicle fusion kinetics in neurons and endocrine cells. The exact mechanisms underlying the actions of PMA, however, is often not clear, largely because of the diversity of the DAG/PMA receptors involved in the exocytotic process, which include, most notably, various isoforms of protein kinase C (PKC). In this study, the roles of PKC{alpha} in PMA-mediated regulation of exocytosis were investigated by over-expressing wild-type PKC{alpha} (wt-PKC{alpha}) or dominant negative PKC{alpha} (dn-PKC{alpha}). Amperometric measurements based on carbon fiber microelectrodes demonstrated that PKC{alpha} has a key role in the PMA-mediated facilitation of exocytosis and vesicle fusion in neuroendocrine PC12 cells.
- OSTI ID:
- 21255920
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 380, Issue 2; Other Information: DOI: 10.1016/j.bbrc.2009.01.105; PII: S0006-291X(09)00129-6; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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