MicroRNA-146a modulates human bronchial epithelial cell survival in response to the cytokine-induced apoptosis
- Pulmonary, Critical Care, Sleep and Allergy Medicine, Department of Internal Medicine, University of Nebraska Medical Center, 985885 Nebraska Medical Center, Omaha, NE 68198-5885 (United States)
MicroRNA plays an important role in cell differentiation, proliferation and cell death. The current study found that miRNA-146a was up-regulated in human bronchial epithelial cells (HBECs) in response to stimulation by TGF-ss1 plus cytomix (a mixture of IL-1ss, IFN-{gamma} and TNF-{alpha}). TGF-ss1 plus cytomix (TCM) induced apoptosis in HBECs (3.4 {+-} 0.6% of control vs 83.1 {+-} 4.0% of TCM treated cells, p < 0.01), and this was significantly blocked by the miRNA-146a mimic (8.8 {+-} 1.5%, p < 0.01). In contrast, a miRNA-146a inhibitor had only a modest effect on cell survival but appeared to augment the induction of epithelial-mesenchymal transition (EMT) in response to the cytokines. The MicroRNA-146a mimic appears to modulate HBEC survival through a mechanism of up-regulating Bcl-XL and STAT3 phosphorylation, and by this mechanism it could contribute to tissue repair and remodeling.
- OSTI ID:
- 21255910
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 380, Issue 1; Other Information: DOI: 10.1016/j.bbrc.2009.01.066; PII: S0006-291X(09)00106-5; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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