(-)-Epigallocatechin gallate inhibition of osteoclastic differentiation via NF-{kappa}B
- Graduate Institute of Pharmaceutical Sciences, College of Pharmacy, Kaohsiung Medical University, No. 100 Shih-Chuan 1st Road, 807 Kaohsiung, Taiwan (China)
- Orthopaedic Research Center, Kaohsiung Medical University, Kaohsiung, Taiwan (China)
People who regularly drink tea have been found to have a higher bone mineral density (BMD) and to be at less risk of hip fractures than those who do not drink it. Green tea catechins such as (-)-epigallocatechin gallate (EGCG) have been reported to increase osteogenic functioning in mesenchymal stem cells. However, its effect on osteoclastogenesis remains unclear. In this study, we investigated the effect of EGCG on RANKL-activation osteoclastogenesis and NF-{kappa}B in RAW 264.7, a murine preosteoclast cell line. EGCG (10-100 {mu}M) significantly suppressed the RANKL-induced differentiation of osteoclasts and the formation of pits in murine RAW 264.7 cells and bone marrow macrophages (BMMs). EGCG appeared to target osteoclastic differentiation at an early stage but had no cytotoxic effect on osteoclast precursors. In addition, it significantly inhibited RANKL-induced NF-{kappa}B transcriptional activity and nuclear translocation. We conclude that EGCG inhibits osteoclastogenesis through its activation of NF-{kappa}B.
- OSTI ID:
- 21255896
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 379, Issue 4; Other Information: DOI: 10.1016/j.bbrc.2009.01.007; PII: S0006-291X(09)00019-9; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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