Analysis of methanogenic activity in a thermophilic-dry anaerobic reactor: Use of fluorescent in situ hybridization
- Department of Chemical Engineering, Food and Environmental Technologies, Faculty of Marine and Environmental Sciences, University of Cadiz, Campus Rio San Pedro s/n, 11510-Puerto Real, Cadiz (Spain)
Methanogenic activity in a thermophilic-dry anaerobic reactor was determined by comparing the amount of methane generated for each of the organic loading rates with the size of the total and specific methanogenic population, as determined by fluorescent in situ hybridization. A high correlation was evident between the total methanogenic activity and retention time [-0.6988Ln(x) + 2.667] (R{sup 2} 0.8866). The total methanogenic activity increased from 0.04 x 10{sup -8} mLCH{sub 4} cell{sup -1}day{sup -1} to 0.38 x 10{sup -8} mLCH{sub 4} cell{sup -1}day{sup -1} while the retention time decreased, augmenting the organic loading rates. The specific methanogenic activities of H{sub 2}-utilizing methanogens and acetate-utilizing methanogens increased until they stabilised at 0.64 x 10{sup -8} mLCH{sub 4} cell{sup -1}day{sup -1} and 0.33 x 10{sup -8} mLCH{sub 4} cell{sup -1}day{sup -1}, respectively. The methanogenic activity of H{sub 2}-utilizing methanogens was higher than acetate-utilizing methanogens, indicating that maintaining a low partial pressure of hydrogen does not inhibit the acetoclastic methanogenesis or the anaerobic process.
- OSTI ID:
- 21217150
- Journal Information:
- Waste Management, Vol. 29, Issue 3; Other Information: DOI: 10.1016/j.wasman.2008.08.010; PII: S0956-053X(08)00284-5; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0956-053X
- Country of Publication:
- United States
- Language:
- English
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