Gene trapping identifies a putative tumor suppressor and a new inducer of cell migration
- Department of Molecular Hematology, University of Frankfurt Medical School, 60590 Frankfurt am Main (Germany)
- Department of Molecular Cell and Aging Research, IUF at University of Duesseldorf gGmbH, Auf'm Hennekamp 50, 40225 Duesseldorf (Germany)
Tumor necrosis factor alpha (TNF{alpha}) is a pleiotropic cytokine involved in apoptotic cell death, cellular proliferation, differentiation, inflammation, and tumorigenesis. In tumors it is secreted by tumor associated macrophages and can have both pro- and anti-tumorigenic effects. To identify genes regulated by TNF{alpha}, we performed a gene trap screen in the mammary carcinoma cell line MCF-7 and recovered 64 unique, TNF{alpha}-induced gene trap integration sites. Among these were the genes coding for the zinc finger protein ZC3H10 and for the transcription factor grainyhead-like 3 (GRHL3). In line with the dual effects of TNF{alpha} on tumorigenesis, we found that ZC3H10 inhibits anchorage independent growth in soft agar suggesting a tumor suppressor function, whereas GRHL3 strongly stimulated the migration of endothelial cells which is consistent with an angiogenic, pro-tumorigenic function.
- OSTI ID:
- 21217115
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 376, Issue 4; Other Information: DOI: 10.1016/j.bbrc.2008.09.070; PII: S0006-291X(08)01827-5; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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