Control of the blue fluorescent protein with advanced evolutionary pulse shaping
- Center for Ultrafast Optical Science, University of Michigan, 2200 Bonisteel Boulevard, Ann Arbor, MI 48109-2099 (United States)
- Institute of Physics, University of Tartu, Riia 142, 51014 Tartu (Estonia)
- Michigan Nanotechnology Institute for Medicine and Biological Sciences, 9220 MSRB III, 1150 West Medical Center Drive, SPC 5648, Ann Arbor, MI 48109-5408 (United States)
We demonstrate optical coherent control of the two-photon fluorescence of the blue fluorescent protein (BFP), which is of interest in investigations of protein-protein interactions. In addition to biological relevance, BFP represents an interesting target for coherent control from a chemical perspective due to its many components of highly nonexponential fluorescence decay and low quantum yield resulting from excited state isomerization. Using a genetic algorithm with a multiplicative (rather than ratiometric) fitness parameter, we are able to control the ratio of BFP fluorescence to second-harmonic generation without a considerable drop in the maximized signal. The importance of linear chirp and power-scaling on the discrimination process is investigated in detail.
- OSTI ID:
- 21217114
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 376, Issue 4; Other Information: DOI: 10.1016/j.bbrc.2008.09.075; PII: S0006-291X(08)01823-8; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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