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Title: Three-Dimensional Visualization of a Human Chromosome Using Coherent X-Ray Diffraction

Abstract

Coherent x-ray diffraction microscopy is a lensless phase-contrast imaging technique with high image contrast. Although electron tomography allows intensive study of the three-dimensional structure of cellular organelles, it has inherent difficulty with thick objects. X rays have the unique benefit of allowing noninvasive analysis of thicker objects and high spatial resolution. We observed an unstained human chromosome using coherent x-ray diffraction. The reconstructed images in two or three dimensions show an axial structure, which has not been observed under unstained conditions.

Authors:
;  [1];  [2]; ;  [3]
  1. RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148 (Japan)
  2. Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871 (Japan)
  3. Cellular Dynamics Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198 (Japan)
Publication Date:
OSTI Identifier:
21180106
Resource Type:
Journal Article
Journal Name:
Physical Review Letters
Additional Journal Information:
Journal Volume: 102; Journal Issue: 1; Other Information: DOI: 10.1103/PhysRevLett.102.018101; (c) 2009 The American Physical Society; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0031-9007
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; CELL CONSTITUENTS; HUMAN CHROMOSOMES; IMAGES; MICROSCOPY; SPATIAL RESOLUTION; THREE-DIMENSIONAL CALCULATIONS; TOMOGRAPHY; X-RAY DIFFRACTION

Citation Formats

Nishino, Yoshinori, Ishikawa, Tetsuya, Takahashi, Yukio, Imamoto, Naoko, and Maeshima, Kazuhiro. Three-Dimensional Visualization of a Human Chromosome Using Coherent X-Ray Diffraction. United States: N. p., 2009. Web. doi:10.1103/PHYSREVLETT.102.018101.
Nishino, Yoshinori, Ishikawa, Tetsuya, Takahashi, Yukio, Imamoto, Naoko, & Maeshima, Kazuhiro. Three-Dimensional Visualization of a Human Chromosome Using Coherent X-Ray Diffraction. United States. https://doi.org/10.1103/PHYSREVLETT.102.018101
Nishino, Yoshinori, Ishikawa, Tetsuya, Takahashi, Yukio, Imamoto, Naoko, and Maeshima, Kazuhiro. 2009. "Three-Dimensional Visualization of a Human Chromosome Using Coherent X-Ray Diffraction". United States. https://doi.org/10.1103/PHYSREVLETT.102.018101.
@article{osti_21180106,
title = {Three-Dimensional Visualization of a Human Chromosome Using Coherent X-Ray Diffraction},
author = {Nishino, Yoshinori and Ishikawa, Tetsuya and Takahashi, Yukio and Imamoto, Naoko and Maeshima, Kazuhiro},
abstractNote = {Coherent x-ray diffraction microscopy is a lensless phase-contrast imaging technique with high image contrast. Although electron tomography allows intensive study of the three-dimensional structure of cellular organelles, it has inherent difficulty with thick objects. X rays have the unique benefit of allowing noninvasive analysis of thicker objects and high spatial resolution. We observed an unstained human chromosome using coherent x-ray diffraction. The reconstructed images in two or three dimensions show an axial structure, which has not been observed under unstained conditions.},
doi = {10.1103/PHYSREVLETT.102.018101},
url = {https://www.osti.gov/biblio/21180106}, journal = {Physical Review Letters},
issn = {0031-9007},
number = 1,
volume = 102,
place = {United States},
year = {Fri Jan 09 00:00:00 EST 2009},
month = {Fri Jan 09 00:00:00 EST 2009}
}