Efficiency Of The Photodynamic Therapy Using Gold Nanoparticles (np-Au) And PpIX Induced And Not Induced
- Departamento de Morfologia. ENCB-IPN, Mexico, D.F. Carpio y Plan de Ayala S/N. Col. Sto. Tomas. Mexico, D.F. C.P. 11340 (Mexico)
- Universidad de Hermosillo, Hermosillo, Sonora (Mexico)
- Central de Microscopia. ENCB-IPN, Mexico, D.F. (Mexico)
- CICATA-IPN, Unidad Legaria, Mexico, D.F. (Mexico)
- Depto. de Fisica, CINVESTAV-IPN, Unidad Zacatenco, Mexico, D.F. (Mexico)
The use of gold nanoparticles (np-Au) to eliminate cancer has proved to be very effective due to the fact that cancerous cells accumulate it 600% more than healthy cells. In addition they have a high capacity of absorption and dispersion of light. Therefore, the effectiveness of photodynamic therapy (PDT) could be improved by the simultaneous use of np-Au and photosensitizes (Ps), emphasizing the high efficiency of the PDT to diagnose and to treat pre-malignant and malignant processes. The aim of this work was to determine the efficiency of PDT using np-Au and protoporphyrin IX (PpIX) induced and not induced by the {delta}-aminolevulinic acid (ALA). It were found the conditions of synthesis of hydrosoluble np-Au, and were characterized by transmission electronic microscopy (TEM) and UV-VIS spectroscopy. It was realized a kinetic by TEM to determine the cellular incorporation time of np-Au, the maximum incorporation of np-Au was of 16 h. PDT was applied using different doses of np-Au and photosensitizers. It was observed that the use of PDT simultaneously with np-Au did not increase the mortality of HeLa cells. In the case of C33, when PpIX not induced is used as photosensitizer simultaneously with np-Au, the mortality increased 20%.
- OSTI ID:
- 21149287
- Journal Information:
- AIP Conference Proceedings, Vol. 1032, Issue 1; Conference: 10. Mexican symposium on medical physics, Mexico City (Mexico), 17-19 Mar 2008; Other Information: DOI: 10.1063/1.2979296; (c) 2008 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA); ISSN 0094-243X
- Country of Publication:
- United States
- Language:
- English
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