DC-SIGN mediates avian H5N1 influenza virus infection in cis and in trans
- Department of Biotechnology and Laboratory Science in Medicine, School of Medicine, National Yang-Ming University, Taipei 112, Taiwan (China)
- Division of Preventive Medicine, Institute of Public Health, School of Medicine, National Yang-Ming University, Taipei 112, Taiwan (China)
- Vaccine Research and Development Center, National Health Research Institutes, Taiwan (China)
- Institute of Anatomy and Cell Biology, School of Medicine, National Yang-Ming University, Taipei 112, Taiwan (China)
- AIDS Prevention and Research Center, School of Medicine, National Yang-Ming University, Taipei 112, Taiwan (China) and Division of Preventive Medicine, Institute of Public Health, School of Medicine, National Yang-Ming University, Taipei 112, Taiwan (China)
DC-SIGN, a C-type lectin receptor expressed in dendritic cells (DCs), has been identified as a receptor for human immunodeficiency virus type 1, hepatitis C virus, Ebola virus, cytomegalovirus, dengue virus, and the SARS coronavirus. We used H5N1 pseudotyped and reverse-genetics (RG) virus particles to study their ability to bind with DC-SIGN. Electronic microscopy and functional assay results indicate that pseudotyped viruses containing both HA and NA proteins express hemagglutination and are capable of infecting cells expressing {alpha}-2,3-linked sialic acid receptors. Results from a capture assay show that DC-SIGN-expressing cells (including B-THP-1/DC-SIGN and T-THP-1/DC-SIGN) and peripheral blood dendritic cells are capable of transferring H5N1 pseudotyped and RG virus particles to target cells; this action can be blocked by anti-DC-SIGN monoclonal antibodies. In summary, (a) DC-SIGN acts as a capture or attachment molecule for avian H5N1 virus, and (b) DC-SIGN mediates infections in cis and in trans.
- OSTI ID:
- 21143840
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 373, Issue 4; Other Information: DOI: 10.1016/j.bbrc.2008.06.078; PII: S0006-291X(08)01222-9; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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