Vitisin A inhibits adipocyte differentiation through cell cycle arrest in 3T3-L1 cells

Journal Article · Fri Jul 18 00:00:00 EDT 2008 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2008.04.188· OSTI ID:21143771

Kim, Soon-hee ^[1]; Park, Hee-Sook; Lee, Myoung-su ^[1]; Cho, Yong-Jin ^[1]; Kim, Young-Sup ^[2]; Hwang, Jin-Taek; Sung, Mi Jeong ^[1]; Kim, Myung Sunny ^[1]; Kwon, Dae Young ^[1]

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1. Korea Food Research Institute, Functional Food Research Center, San 46-1, Baekhyun-dong, Bundang-ku, Songnam, Kyungki-do 463-746 (Korea, Republic of)
2. Korea Research Institute of Chemical Technology, Daejun (Korea, Republic of)

Inhibition of adipocyte differentiation is one approach among the anti-obesity strategies. This study demonstrates that vitisin A, a resveratrol tetramer, inhibits adipocyte differentiation most effectively of 18 stilbenes tested. Fat accumulation and PPAR{gamma} expression were decreased by vitisin A in a dose-dependent manner. Vitisin A significantly inhibited preadipocyte proliferation and consequent differentiation within the first 2 days of treatment, indicating that the anti-adipogenic effect of vitisin A was derived from anti-proliferation. Based on cell cycle analysis, vitisin A blocked the cell cycle at the G1-S phase transition, causing cells to remain in the preadipocyte state. Vitisin A increased p21 expression, while the Rb phosphorylation level was reduced. Therefore, vitisin A seems to induce G1 arrest through p21- and consequent Rb-dependent suppression of transcription. On the other hand, ERK and Akt signaling pathways were not involved in the anti-mitotic regulation by vitisin A. Taken together, these results suggest that vitisin A inhibits adipocyte differentiation through preadipocyte cell cycle arrest.

• OSTI ID: 21143771
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 372, Issue 1; Other Information: DOI: 10.1016/j.bbrc.2008.04.188; PII: S0006-291X(08)00876-0; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CELL CYCLE
INHIBITION
METABOLIC DISEASES
MONOCLINIC LATTICES
PHASE TRANSFORMATIONS
PHOSPHORUS 21
PHOSPHORYLATION
PROLIFERATION
STILBENE
TRANSCRIPTION